TMT labeled comparative proteomic analysis reveals spleen active immune responses during Clostridium perfringens type C infected piglet diarrhea
文献类型： 外文期刊
作者： Wang, Xiaoli 1 ; Huang, Xiaoyu 1 ; Yang, Qiaoli 1 ; Yan, Zunqiang 1 ; Wang, Pengfei 1 ; Gao, Xiaoli 1 ; Luo, Ruirui 1 ; Gun, Shuangbao 1 ;
作者机构： 1.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou, Gansu, Peoples R China
2.Guizhou Inst Prataculture, Guizhou Acad Agr Sci, Guiyang, Peoples R China
3.Gansu Res Ctr Swine Prod Engn & Technol, Lanzhou, Gansu, Peoples R China
关键词： TMT labeling; Proteome; Clostridium perfringens type C; Piglet diarrhea; Immune Response; Spleen
期刊名称：PEERJ （ 影响因子：3.061； 五年影响因子：3.537 ）
ISSN： 2167-8359
年卷期： 2022 年 10 卷
页码：
收录情况： SCI
摘要： Background. Clostridium perfringens (C. perfringens) type C is the principal pathogenic clostridia of swine, frequently causing hemorrhagic diarrhea, even necrotic enteritis in piglets, leading to severe economic loss for swine industries worldwide. However, there are no specific and effective prevention measures. Therefore, clarifying the molecular mechanisms of hosts against pathogenesis infection is very important to reduce the incidence of C. perfringens type C infected piglet diarrhea disease. Methods. We performed an TMT labeling-based quantitative spleen proteomic analysis of the control group (SC), tolerance group (SR) and susceptible group (SS) to identify the differentially expressed proteins (DEPs), and screened potential molecular markers of piglet spleen tissues in response to C. perfringens type C infection. Results. In this study, a total of 115, 176 and 83 DEPs were identified in SR vs SC, SS vs SC, and SR vs SC, respectively, which may play the important regulatory roles in the process of piglet spleens in response toC. perfringens type C-infected diarrhea diseases. GO enrichment analysis revealed that the DEPs were mostly significantly enriched in acute inflammatory response, defense response, antimicrobial response, transporter activity, cellular metabolic process and so on, and KEGG pathway enrichment analysis showed that the significantly enriched immune related pathways of the PPAR signaling pathway, IL-17 signaling pathway, antigen processing and presentation, which hints at the immune defense process of piglet spleen against C. perfringens infection. This study helps to elucidate the protein expressional pattern of piglet spleen against C. perfringens type C-infected diarrhea disease, which can contribute to the prevention and control for pig diarrhea disease and the further development of diarrhea resistant pig breeding.
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