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Physiological and transcriptional responses of two contrasting Populus clones to nitrogen stress

文献类型： 外文期刊

作者： Wang, Xiaoli 1 ; Li, Xiaodong 2 ; Zhang, Sheng 3 ; Korpelainen, Helena 4 ; Li, Chunyang 5 ;

作者机构： 1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Inst Prataculture, Guiyang 550006, Guizhou, Peoples R China

3.Chinese Acad Sci, Inst Mt Hazards & Environm, Chengdu 610041, Peoples R China

4.Univ Helsinki, Viikki Plant Sci Ctr, Dept Agr Sci, POB 27, FI-00014 Helsinki, Finland

5.Zhejiang A&F Univ, Nurturing Stn, State Key Lab Subtrop Silviculture, Linan 311300, Zhejiang, Peoples R China

关键词： gene expression profiling;miRNAs

期刊名称：TREE PHYSIOLOGY （ 影响因子：4.196； 五年影响因子：4.727 ）

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收录情况： SCI

摘要： The aim of this study was to reveal mechanisms responsible for nitrogen (N) stress in two contrasting Populus clones. Leaves of Nanlin 1388 (N stress-insensitive clone hybrids of Populus deltoides Bart.CV.aEuro...xaEuro...Populus euramericana (Dode) Guineir CV) and Nanlin 895 (N stress-sensitive clone hybrids of Populus deltoides Bart.CV.aEuro...xaEuro...Populus euramericana (Dode) Guineir CV) were harvested and analyzed. Different responses visible in photosynthesis, N and carbon contents, physiological traits, and chlorophyll were observed. The Solexa/Illumina's digital gene expression system was used to investigate differentially expressed miRNAs and mRNAs under N stress. Target profiling, and biological network and function analyses were also performed. Randomly selected mRNAs and miRNAs were validated by quantitative reverse transcription polymerase chain reaction. In all, 110 Nanlin 1388 and 122 Nanlin 895 miRNAs were differentially expressed, among which 34 and 23 miRNAs were newly found in the two clones, respectively. Under N stress, a total of 329 and 98 mRNAs were regulated in N stress-insensitive and -sensitive clones, respectively. Notably, the miR396 family and its regulated mRNAs were altered in both clones under N stress, while miR646 was regulated only in the N stress-insensitive clone (Nanlin 1388), and miR156, miR319 and miR393 in the N stress-sensitive clone (Nanlin 895). Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses also proved several clone-specific functions and pathways. These findings may be significant for understanding the genetic responses of Populus to N stress.

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