Selection of goat 8-casein derived ACE-inhibitory peptide SQPK and insights into its effect and regulatory mechanism on the function of endothelial cells
文献类型： 外文期刊
作者： Wu, Yulong 1 ; Zhang, Jin 1 ; Mu, Tong 1 ; Zhang, Hong 1 ; Cao, Jianxin 1 ; Li, Huanhuan 1 ; Tang, Honggang 1 ; Chen, Lihong 1 ; Liu, Hongyun 3 ; Xu, Xianrong 2 ; Zhao, Ke 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Food Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Zhejiang, Peoples R China
2.Hangzhou Normal Univ, Sch Publ Hlth, Hangzhou 311121, Zhejiang, Peoples R China
3.Zhejiang Univ, Coll Anim Sci, Key Lab Mol Anim Nutr, Minist Educ, Hangzhou 310058, Zhejiang, Peoples R China
关键词： SQPK; ACE inhibitory peptide; Goat 8-casein; Endothelial cells; Transcriptomic profiling
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2023 年 253 卷
页码：
收录情况： SCI
摘要： The angiotensin I-converting enzyme (ACE)-inhibitory peptide SQPK was selected by in silico digestion and virtual screening from goat beta-casein, and its effect and regulatory mechanism on function of endothelial cells was further evaluated. The results showed that SQPK exhibited relatively good ACE inhibition capacity (IC50 = 452.7 mu g/mL). Treatment with 25 mu g/mL SQPK for 12 h significantly elevated nitric oxide (NO) production, stimulated eNOS expression (p < 0.05) and affected the transcriptomic profiling of EA. Hy926 cells. In particular, SQPK stimulated the expression of genes encoding inflammatory cytokines (CXCL1/2 and IL6) but depressed encoding mesenchymal markers (FN1 and CNN3). Furthermore, SQPK modified the expression of genes involved in endothelial-to-mesenchymal transition (EndMT). Therefore, the selected peptide SQPK may exert potential protective effects on the function of endothelial cells by inhibiting the EndMT.
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