A benzophenoxazine-based NIR fluorescent probe for the detection of hydrogen sulfide and imaging in living cells
文献类型： 外文期刊
作者： Yin, Wenzhu 1 ; Wang, Hongjing 2 ; Ma, Fang 1 ; Xu, Zeyu 3 ; Lu, Yu 1 ; Zhou, Mingxu 1 ; Deng, Bihua 1 ; Wang, Haiyan 1 ; Zhang, Jinqiu 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Natl Res Ctr Engn & Technol Vet Biol, Jiangsu Key Lab Food Qual & Safety,Minist Sci & T, State Key Lab Cultivat Base,Inst Vet Immunol & En, Nanjing 210014, Peoples R China
2.YiLi Normal Univ, Coll Chem & Enviromental Sci, Yining 835000, Peoples R China
3.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
期刊名称：ANALYST （ 影响因子：5.227； 五年影响因子：4.793 ）
ISSN： 0003-2654
年卷期： 2022 年 147 卷 20 期
页码：
收录情况： SCI
摘要： Hydrogen sulfide (H2S) plays a vital role in regulating many important physiological functions. However, H2S-containing industrial wastewater is inevitably pumped into the environment which seriously contaminates water supplies and foodstuffs. So it is crucial to develop a single fluorescent probe for H2S detection with high sensitivity and selectivity. Herein, a colorimetric and turn-on near infrared (NIR) fluorescent probe NRDNP based on benzophenoxazine was designed and synthesized by thiolysis of 2,4-dinitrophenyl (DNP) ether as the specific reaction site strategy to achieve highly specific H2S detection in living systems. The studies demonstrated that the probe NRDNP exhibited excellent sensing performance toward H2S with an about 80-fold NIR fluorescence enhancement, a rapid response within 10 min, excellent sensitivity with a detection limit of 19 nM and good selectivity. Furthermore, the NRDNP is an optical sensor which visually changes in terms of the fluorescence colour/intensity upon sensing gaseous H2S molecules. NRDNP has low cytotoxicity and has been successfully applied in the fluorescence imaging of H2S in living cells, suggesting that it would be an effective tool for H2S detection in living systems.
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