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Construction of a genetic linkage map in Fenneropenaeus chinensis using SNP markers

文献类型： 外文期刊

作者： Zhang, Jian-Yong 1 ; Wang, Wei-Ji 2 ; Kong, Jie 2 ; Wang, Qing-Yin 2 ;

作者机构： 1.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： single nucleotide polymorphisms (SNP);genetic linkage;Fenneropenaeus chinensis

期刊名称：RUSSIAN JOURNAL OF MARINE BIOLOGY （ 影响因子：0.621； 五年影响因子：0.675 ）

ISSN： 1063-0740

年卷期： 2013 年 39 卷 2 期

页码：

收录情况： SCI

摘要： Genetic linkage maps of Fenneropenaeus chinensis were constructed using a "double pseudo-testcross" strategy with 200 single nucleotide polymorphisms (SNPs) markers. This study represents the first SNP genetic linkage map for F. chinensis. The parents and F (1) progeny of 100 individuals were used as mapping populations. 21 genetic linkage groups in the male and female maps were identified. The male linkage map was composed of 115 loci and spanned 879.7 cM, with an average intermarker spacing of 9.4 cM, while the female map was composed of 119 loci and spanned 876.2 cM, with an average intermarker spacing of 8.9 cM. The estimated coverage of the linkage maps was 51.94% for the male and 53.77% for the female, based on two estimates of genome length. The integrated map contains 180 markers distributed in 16 linkage groups, and spans 899.3 cM with an average marker interval of 5.2 cM. This SNP genetic map lays the foundation for future shrimp genomics and genetic breeding studies, especially the discovery of gene or regions for economically important traits in Chinese shrimp.

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