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Study of Ultrasonic-freeze-thaw-cycle Assisted Extraction of Polysaccharide and Phycobiliprotein from Gracilaria lemaneiformis

文献类型： 外文期刊

作者： Liu, Mingqiu 1 ; Yang, Xianqing 1 ; Qi, Bo 1 ; Li, Laihao 1 ; Deng, Jianchao 1 ; Hu, Xiao 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Natl R&D Ctr Aquat Prod Proc, Key Lab Aquat Prod Proc,Minist Agr, Beijing, Peoples R China

关键词： ultrasonic-freeze-thaw-cycle assisted extraction;polysaccharide;phycobiliprotein;Gracilaria lemaneiformis

期刊名称：ADVANCES IN CHEMICAL ENGINEERING III, PTS 1-4

ISSN： 1022-6680

年卷期： 2013 年 781-784 卷

页码：

收录情况： SCI

摘要： Ultrasonic-freeze-thaw-cycle assisted extraction was used to synchronously extract polysaccharide and phycobiliprotein from Gracilaria lemaneiformis. Firstly, different solvents(deionized water, sodium hydroxide, phosphate buffer solution (PBS) and calcium chloride solution) were screened, and PBS (1 mM, pH 6.8) was considered as the best solvent for its OD565 value (0.086+/-0.0003) and OD595 value (0.471+/-0.002), which was significantly higher than any other solvents (p<0.05). Moreover, an L-9 (3)(4) orthogonal test was carried out to optimize extraction conditions, based on the single-factor test. The results indicated that the optimized conditions were fixed as follows: the ratio of solid to liquid 1:25 (w/v), 2 times of freeze-thaw cycles, ultrasound power 320 W and ultrasound time 10 min, which obtained the optimum yield and purity of polysaccharide and phycobiliprotein, 30.54%, 1.36% and 0.296, respectively. As a result, ultrasonic-freeze-thaw-cycle assisted extraction is considered to be an effective, economic and eco-environmental method to extract polysaccharide and phycobiliprotein.

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