您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular characterization and expression of a novel big defensin (Sb-BDef1) from ark shell, Scapharca broughtonii

文献类型： 外文期刊

作者： Li, Meng 1 ; Zhu, Ling 1 ; Zhou, Chun-ya 1 ; Sun, Shan 1 ; Fan, Yan-jun 1 ; Zhuang, Zhi-meng 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： Big defensin;Scapharca broughtonii;Quantitative real-time PCR;Tissue distribution;Gram-negative microbial challenge

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 2020影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2012 年 33 卷 5 期

页码：

收录情况： SCI

摘要： Big defensins, endogenous cysteine-rich antimicrobial peptides (AMPS) with antimicrobial activity and immunomodulatory property, play crucial roles in host defense against various microbial pathogens. A novel big defensin (Sb-BDef1) of ark shell Scapharca broughtonii was identified by expressed sequence tag (EST) and RACE techniques. The Sb-BDef1 cDNA contained an open reading frame (ORF) of 336-bp encoding a polypeptide of 111 amino acids with a putative signal peptide of 21 amino acid residues, followed by a putative propeptide of 11 residues and a putative mature peptide of 79 residues. The mature peptide shared the common features of big defensins, including a high hydrophobic residues region (59%) in the N-terminus, a defensin domain in the C-terminus, which perfectly corresponds to the six conserved disulfide-bonded cysteine residues involved in the formation of the internal disulfide bridges (C1-C5, C2-C4 and C3-C6) in all big defensins from mollusk, horseshoe crab and amphioxus. Quantitative real-time PCR analysis revealed that the expression of Sb-BDef1 transcript was detected in all the tissues examined from normal ark shells, and the temporal expression of Sb-BDef1 mRNA was remarkably up-regulated at 8, 16 h in hemocytes, and at 16, 24 h in hepatopancreas after Vibrio anguillarum-challenge, respectively. These results suggested that Sb-BDef1 was a constitutive and inducible acute-phase protein and should be involved in immune response of Gram-negative microbial infection in ark shell S. broughtonii. (C) 2012 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa. Li, Meng,Zhu, Ling,Zhou, Chun-Ya,Lin, Lin,Fan, Yan-Jun,Zhuang, Zhi-Meng,Li, Meng,Zhou, Chun-Ya. 2012

[2]Isolation and characterization of 48 polymorphic microsatellite markers for the blood clam Scapharca broughtonii (Arcidae). Tian, J. -T.,Liu, Z. -H.,Zhou, L. -Q.,Wu, B.,Liu, P.,Yang, A. -G.,Tian, J. -T.. 2012

[3]A manganese superoxide dismutase (MnSOD) from ark shell, Scapharca broughtonii: Molecular characterization, expression and immune activity analysis. Zheng, Libing,Wu, Biao,Liu, Zhihong,Tian, Jiteng,Zhou, Liqing,Sun, Xiujun,Yang, Aiguo,Zheng, Libing,Yu, Tao.

[4]Identification and expression analysis on bactericidal permeability-increasing protein (BPI)/lipopolysaccharide-binding protein (LBP) of ark shell, Scapharca broughtonii. Mao, Yuze,Zhou, Chunya,Zhu, Ling,Huang, Yao,Yan, Tingru,Fang, Jianguang,Zhou, Chunya,Huang, Yao,Zhu, Wei.

[5]Ferritin has an important immune function in the ark shell Scapharca broughtonii. Zheng, Libing,Liu, Zhihong,Wu, Biao,Zhou, Liqing,Tian, Jiteng,Sun, Xiujun,Yang, Aiguo,Zheng, Libing,Dong, Yinghui.

[6]Identification and characterization of ostreid herpesvirus 1 associated with massive mortalities of Scapharca broughtonii broodstocks in China. Bai, Changming,Gao, Wenhui,Wang, Chongming,Wang, Qingchen,Huang, Jie,Bai, Changming,Wang, Chongming,Huang, Jie,Yu, Tao,Zhang, Tianwen,Qiu, Zhaoxing.

[7]Selenocompounds in juvenile white sturgeon: Evaluating blood, tissue, and urine selenium concentrations after a single oral dose. Huang, Susie Shih-Yin,Strathe, Anders Bjerring,Fadel, James G.,Hung, Silas S. O.,Wang, Wei-Fang,Deng, Dong-Fang. 2012

[8]Pharmacokinetics, Tissue Distribution, and Elimination of Flumequine in Japanese Seabass (Lateolabrax japonicus) following Intraperitoneal Injection and Oral Gavage. Hu, Lin-lin,Fang, Wen-hong,Zhou, Kai,Xia, Lian-jun,Zhou, Shuai,Lou, Bao,Shi, Hui-lai. 2011

[9]Nibea coibor growth hormone gene: Its phylogenetic significance, microsatellite variation and expression analysis. Xu, Xinping,Xu, Xinping,Zhang, Dianchang,Jiang, Shigui,Li, Jianzhu,Zhang, Dianchang,Shao, Yanqing. 2009

[10]Occurrence and dietary exposure assessment of PFOS and PFOA in cultured Trachinotus ovatus in China. Meng, Di,Qian, Yongzhong,Meng, Di,Han, Gang,Guo, Mengmeng. 2017

[11]Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides). Shi, Yan,Zhu, Xinping,Chen, Kunci,Zhao, Zhe,Zhang, Qiya. 2013

[12]A novel serine protease with clip domain from scallop Chlamys farreri. Zhu, Ling,Song, Linsheng,Zhao, Jiangmin,Li, Chenghua,Xu, Wei,Zhu, Ling,Mao, Yuze. 2008

[13]Ontogeny expression of ghrelin, neuropeptide Y and cholecystokinin in blunt snout bream, Megalobrama amblycephala. Ping, H. -C.,Feng, K.,Zhang, G. -R.,Wei, K. -J.,Wang, W. -M.,Zou, G. -W.. 2014

[14]A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action. Zhang, Linbao,Yang, Dinglong,Wang, Qing,Wu, Huifeng,Pei, Dong,Cong, Ming,Li, Fei,Ji, Chenglong,Zhao, Jianmin,Zhang, Linbao,Yang, Dinglong,Wang, Qing,Wu, Huifeng,Pei, Dong,Cong, Ming,Li, Fei,Ji, Chenglong,Zhao, Jianmin,Zhang, Linbao,Yang, Dinglong,Yuan, Zeyi.

[15]Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense. Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Liu, Xiaoqian.

[16]Transplacental transfer of phenyltins from a pregnant Dall's porpoise (Phocoenoides dalli) to her fetus. Yang, Jian,Harino, Hinoya,Miyazaki, Nobuyuki.

[17]Pharmacokinetics of sulfamethoxazole and trimethoprim in Pacific white shrimp, Litopenaeus vannamei, after oral administration of single-dose and multiple-dose. Ma, Rongrong,Wang, Yuan,Zou, Xiong,Sun, Beibei,Fang, Wenhong,Fu, Guihong,Ma, Rongrong,Hu, Kun,Yang, Xianle.

[18]Molecular characterization, expression and function analysis of a five-domain Kazal-type serine proteinase inhibitor from pearl oyster Pinctada fucata. Zhang, Dianchang,Ma, Jianjun,Jiang, Shigui. 2014

[19]Characterization, molecular cloning, and expression analysis of Ecsit in the spinyhead croaker, Collichthys lucidus. Song, W.,Jiang, K. J.,Zhang, F. Y.,Wang, J.,Ma, L. B.. 2016

[20]Characterization and expression analysis of the prophenoloxidase activating factor from the mud crab Scylla paramamosain. Wang, J.,Jiang, K. J.,Zhang, F. Y.,Song, W.,Zhao, M.,Wei, H. Q.,Meng, Y. Y.,Ma, L. B.,Wang, J.,Zhao, M.,Wei, H. Q.,Meng, Y. Y.. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台