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Transcriptome analysis revealed the mechanism of Luciobarbus capito (L. capito) adapting high salinity: Antioxidant capacity, heat shock proteins, immunity

文献类型： 外文期刊

作者： Shang, Xinchi 1 ; Xu, Wei 1 ; Zhang, Ying 1 ; Sun, Qingsong 3 ; Li, Zhengwei 5 ; Geng, Longwu 1 ; Teng, Xiaohua 4 ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Rd 43 Songfa, Harbin 150070, Peoples R China

2.Key Lab Cold Water Fish Germplasm Resources & Mult, Harbin 150070, Heilongjiang, Peoples R China

3.Jilin Agr Sci & Technol Univ, Key Lab Prevent Vet Med Jilin Prov, 77 Hanlin Rd, Jilin 132101, Peoples R China

4.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China

5.D Heilongjiang Prov Aquat Anim Resources Conservat, Wuchang, Peoples R China

关键词： Transcriptome analysis; Salinity; Oxidative stress; Signal pathway; Hematological parameter

期刊名称：MARINE POLLUTION BULLETIN （ 影响因子：5.8； 五年影响因子：6.5 ）

ISSN： 0025-326X

年卷期： 2023 年 192 卷

页码：

收录情况： SCI

摘要： Salinity has a significant influence on the physiology of freshwater aquatic organisms. However, there are few studies on the hematology and immunology of freshwater fish under high salinity. In the current study, we aimed to analyze the adaptive effect of salt stress on L. capito spleen immune function and hematology using tran-scriptomic analysis. We replicated a L. capito acute salinity stress model, and collected blood and spleens from freshwater and saltwater fish. It was found that salinity affected significantly the numbers of leukocytes, lym-phocytes, neutrophils, and red blood cells, as well as the content of haemoglobin. Salt treatment resulted in significant increase in the expression of HSP70, HSP90, CAT, SOD, and GPX1 genes in L. capito spleens. Tran-scriptomic analysis revealed a total of 546 differentially expressed genes (DEGs) in spleens, including 224 regulated DEGs and 322 down-regulated DEGs. In addition, GO enrichment analysis revealed immune system process, multicellular organismal process, and biological regulation of genes with the most differences in bio-logical processes. KEGG enrichment analysis showed that the regulation of lipolysis in adipocyte, FoxO signaling pathway, Hematopoietic cell lineage signaling pathway, and HIF-1 signaling pathway were significantly enriched. L. capito adapted oxidative to high salinity through FoxO signaling pathway and immune to high salinity through Hematopoietic cell lineage signaling pathway. At the same time, we selected 10 DEGs for qRT-PCR detection, and the results showed that the qRT-PCR results were consistent with our RNA-Seq results, indicating that transcriptome sequencing was accurate and reliable. In conclusion, our results demonstrated that the improvement of antioxidant capacity, heat shock protein and immunity are involved in the molecular mechanism of L. capito adapting to high salinity. Our findings provided a rationale for further study on high salinity adaptation and related enrichment pathways.

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