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Identification and Characterization of High-Molecular-Weight Proteins Secreted by Plasmodiophora brassicae That Suppress Plant Immunity

文献类型： 外文期刊

作者： Feng, Yanqun 1 ; Yang, Xiaoyue 1 ; Cai, Gaolei 5 ; Wang, Siting 1 ; Liu, Pingu 1 ; Li, Yan 1 ; Chen, Wang 1 ; Li, Wei 4 ;

作者机构： 1.Yangtze Univ, Coll Agr, MARA Key Lab Sustainable Crop Prod Middle Reaches, Coconstruct Minist & Prov, Jingzhou 434025, Peoples R China

2.Yangtze Univ, Coll Agr, Engn Res Ctr Ecol & Agr Use Wetland, Minist Educ, Jingzhou 434025, Peoples R China

3.Yangtze Univ, Coll Agr, Hubei Collaborat Innovat Ctr Grain Ind, Jingzhou 434025, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

5.Shiyan Acad Agr Sci, Inst Plant Protect, Shiyan 442000, Peoples R China

关键词： clubroot disease; secreted protein; PTI; SA signaling pathway; JA signaling pathway

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN：

年卷期： 2024 年 10 卷 7 期

页码：

收录情况： SCI

摘要： Plasmodiophora brassicae is an obligate intracellular parasitic protist that causes clubroot disease on cruciferous plants. So far, some low-molecular-weight secreted proteins from P. brassicae have been reported to play an important role in plant immunity regulation, but there are few reports on its high-molecular-weight secreted proteins. In this study, 35 putative high-molecular-weight secreted proteins (>300 amino acids) of P. brassicae (PbHMWSP) genes that are highly expressed during the infection stage were identified using transcriptome analysis and bioinformatics prediction. Then, the secretory activity of 30 putative PbHMWSPs was confirmed using the yeast signal sequence trap system. Furthermore, the genes encoding 24 PbHMWSPs were successfully cloned and their functions in plant immunity were studied. The results showed that ten PbHMWSPs could inhibit flg22-induced reactive oxygen burst, and ten PbHMWSPs significantly inhibited the expression of the SA signaling pathway marker gene PR1a. In addition, nine PbHMWSPs could inhibit the expression of a marker gene of the JA signaling pathway. Therefore, a total of 19 of the 24 tested PbHMWSPs played roles in suppressing the immune response of plants. Of these, it is worth noting that PbHMWSP34 can inhibit the expression of JA, ET, and several SA signaling pathway marker genes. The present study is the first to report the function of the high-molecular-weight secreted proteins of P. brassicae in plant immunity, which will enrich the theory of interaction mechanisms between the pathogens and plants.

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