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Nested PCR detection of abalone shriveling syndrome-associated virus in China

文献类型： 外文期刊

作者： Jiang, Jing-Zhe 1 ; Liang, Ya-Yu 2 ; Luo, Li-Juan 1 ; Guo, Zhi-Xun 1 ; Zhuang, Jun 3 ; Liu, Guang-Feng 1 ; Su, You-Lu; 1 ;

作者机构： 1.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

2.S China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China

3.Fujian Agr & Forestry Univ, Key Lab Plant Virol Fujian Prov, Inst Plant Virol, Fuzhou 350002, Peoples R China

关键词： Abalone;Haliotis diversicolor;Abalone shriveling syndrome-associated virus;Nested PCR

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2012 年 184 卷 1-2 期

页码：

收录情况： SCI

摘要： Haliotis diversicolor (small abalone) is an economic seafood found off the Southern coast of China. Since 1999, the cultured abalone yields in China have been affected severely by continual outbreaks of a fatal epidemic disease caused by abalone shriveling syndrome associated virus (AbSV), a double-stranded DNA virus. Although the pathogenicity and genome of AbSV have been ascertained, the epidemiology of AbSV infection remains to be investigated. In the present study, four pairs of AbSV-specific primers were designed on the basis of open reading frame (ORF)24 and ORF25 sequences in the AbSV genome. Two nested PCR detection methods were established by optimization of the annealing temperatures of primers. The results showed that the specificity of primers for AbSV detection could not be interfered with by the host genome and other aquaculture species or viruses. The detection limits of the two methods were about 10 copies of recombinant plasmid containing AbSV genes in 20 mu L reaction mixture. The results of detection of the AbSV epidemic showed that AbSV was still present in juvenile abalones in some farms along the Southern coast of China (Fujian and Guangdong). (C) 2012 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Quantitative PCR detection for abalone shriveling syndrome-associated virus. Jiang Jing-Zhe,Zhang Han,Guo Zhi-Xun,Liu Guang-Feng,Su You-Lu,Wang Jiang-Yong,Zhu Zhen-Ni,Liang Ya-Yu,Zhang Han. 2012

[2]Detection of abalone shrivelling syndrome-associated virus using loop-mediated isothermal amplification. Jiang, J-Z,Yang, R.,Zhang, H.,Liu, G-F,Su, Y-L,Wang, J-Y,Yang, R.,Zhang, H.,Zhu, Z-N,Wu, E-Y. 2014

[3]Dietary selenium requirement and its toxicity in juvenile abalone Haliotis discus hannai Ino. Wang, Weifang,Mai, Kangsen,Zhang, Wenbing,Xu, Wei,Ai, Qinghui,Liufu, Zhiguo,Li, Huitao,Wang, Weifang,Li, Huitao.

[4]Genetic mapping and quantitative trait loci analysis of growth-related traits in the small abalone Haliotis diversicolor using restriction-site-associated DNA sequencing. Ren, Peng,You, Weiwei,Huang, Zekun,Guo, Qing,Chen, Nan,He, Peiru,Ke, Caihuan,Ren, Peng,Huang, Zekun,Guo, Qing,Chen, Nan,He, Peiru,Ke, Jinwei,Ke, Caihuan,Peng, Wenzhu,Peng, Wenzhu,Gwo, Jin-Chywan.

[5]Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization. Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,You, Weiwei,Ke, Caihuan,Zhang, Xiaojun,Jiang, Likun,Chen, Baohua,Mahboob, Shahid,Al-Ghanim, Khalid A.,Mahboob, Shahid.

[6]Identification and involvement of ferritin in the response to pathogen challenge in the abalone, Haliotis diversicolor. He, Jian,Jiang, Jingzhe,Gu, Lu,Zhao, Manman,Wang, Ruixuan,Ye, Lingtong,Yao, Tuo,Wang, Jiangyong,Gu, Lu,Zhao, Manman.

[7]Functional annotation of an expressed sequence tag library from Haliotis diversicolor and analysis of its plant-like sequences. Jiang, Jing-Zhe,Guo, Zhi-Xun,Su, You-Lu,Wang, Rui-Xuan,Wang, Jiang-Yong,Zhang, Wei,Cai, Chen-Chen.

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