Transcriptomic Analysis Provides Novel Insights into the Heat Stress-Induced Response in Codonopsis tangshen
文献类型： 外文期刊
作者： Jiang, Xiaogang 1 ; Wassie, Misganaw 2 ; Zhou, Wuxian 1 ; Wang, Hua 1 ; You, Jinwen 1 ; Ma, Guangjing 2 ; Zhang, Meide 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Chinese Herbal Med, Key Lab Biol & Cultivat Herb Med, Minist Agr & Rural Affairs, Enshi 445000, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, CAS Key Lab Plant Germplasm Enhancement & Specialt, Wuhan Bot Garden, Wuhan 430074, Peoples R China
关键词： Codonopsis tangshen; heat-stress; heat-responsive genes; RNA-sequencing; transcriptome
期刊名称：LIFE-BASEL （ 影响因子：3.2； 五年影响因子：3.2 ）
ISSN：
年卷期： 2023 年 13 卷 1 期
页码：
收录情况： SCI
摘要： Codonopsis tangshen Oliv (C. tangshen) is a valuable traditional Chinese medicinal herb with tremendous health benefits. However, the growth and development of C. tangshen are seriously affected by high temperatures. Therefore, understanding the molecular responses of C. tangshen to high-temperature stress is imperative to improve its thermotolerance. Here, RNA-Seq analysis was performed to investigate the genome-wide transcriptional changes in C. tangshen in response to short-term heat stress. Heat stress significantly damages membrane stability and chlorophyll biosynthesis in C. tangshen, as evidenced by pronounced malonaldehyde (MDA), electrolyte leakage (EL), and reduced chlorophyll content. Transcriptome analysis showed that 2691 differentially expressed genes (DEGs) were identified, including 1809 upregulated and 882 downregulated. Functional annotations revealed that the DEGs were mainly related to heat shock proteins (HSPs), ROS-scavenging enzymes, calcium-dependent protein kinases (CDPK), HSP-HSP network, hormone signaling transduction pathway, and transcription factors such as bHLHs, bZIPs, MYBs, WRKYs, and NACs. These heat-responsive candidate genes and TFs could significantly regulate heat stress tolerance in C. tangshen. Overall, this study could provide new insights for understanding the underlying molecular mechanisms of thermotolerance in C. tangshen.
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