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Determination of Photosynthetic Pigments in Sea Water and Marine Sediments by High Performance Liquid Chromatography

文献类型： 外文期刊

作者： Jiang Tao 1 ; Cong Min 1 ; Gan Ju-Li 2 ; Mu Qing-Lin 3 ; Fang Jie 3 ; Teng De-Qiang 1 ; Lu Song-Hui 1 ;

作者机构： 1.Jinan Univ, Res Ctr Harful Algae & Aquat Environm, Guangzhou 510632, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

3.Zhejiang Zhoushan Marine Ecol Environm Monitoring, Zhoushan 316000, Peoples R China

关键词： Photosynthetic pigments;High performance liquid chromatography;Sea water;Sediments;Marine microalgae

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2012 年 40 卷 4 期

页码：

收录情况： SCI

摘要： An analytical method was developed for the simultaneous determination of 30 photosynthetic pigments by reversed phase high performance liquid chromatography (RP-HPLC). About 1 L of natural seawater was filtrated on a 0.45-mu m GF/F filter. The GF/F filters and marine sediments were extracted in 95% methanol and 95% acetone by ultrasonication, respectively. The separation of pigments extraction was performed on Eclipse XDB C-8 column (150 mmx4.6 mm, 3.5 mu m) at wavelength of 430, 440, 450 nm and at column temperature of 50 degrees C. The mobile phase included three solvents, solvent A, solvent B and solvent B. Solvent A and B were acetonitrile and methanol, respectively. Solvent C was the mixed solution contained 70:30 (V/V) methanol and 28 mmol/L aqueous tetrabutyl ammonium hydroxide-acetate (pH 6.5). The chromatographic process included 2 steps of gradient elution and 1 step of isocratic elution. From 0 to 22 min, the solvent A and B increased from 0 to 25% and 45%, respectively, and solvent C decreased from 100% to 30%, followed by gradient elution to 70% A, 20% B and 10% C for 6 min. Afterwards, the isocratic mode was performed for 10 min and back to the initial conditions. The correlation coefficients for the 23 photosynthetic pigments, of which concentrations were known, ranged from 0.9972 to 0.9998, with detection limits from 0.0305 ng to 0.7740 ng, and recovery from 88.6% to 103.3%.

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