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Differences in ASP1 expression and binding dynamics to queen mandibular pheromone HOB between Apis mellifera and Apis cerana workers reveal olfactory adaptation to colony organization

文献类型： 外文期刊

作者： Wu, Fan 1 ; Liu, Shenyun 1 ; Zhang, Xufeng 2 ; Hu, Han 2 ; Wei, Qiaohong 2 ; Han, Bin 2 ; Li, Hongliang 1 ;

作者机构： 1.China Jiliang Univ, Coll Life Sci, Zhejiang Prov Lab Biometrol & Inspect & Quarantin, Hangzhou 310018, Peoples R China

2.Chinese Acad Agr Sci, Inst Apicultural Res, Minist Agr & Rural Affairs, Key Lab Pollinating Insect Biol, Beijing 100093, Peoples R China

3.Shanxi Agr Univ, Shanxi Acad Agr Sci, Inst Hort Res, Taiyuan 030031, Peoples R China

关键词： ASP1; HOB; Olfactory adaptation; Molecular docking; Fluorescence spectroscopy; Site-directed mutation

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2022 年 217 卷

页码：

收录情况： SCI

摘要： The eastern Apis cerana (Ac) and the western Apis mellifera (Am) are two closely related and most economically valuable honeybee species managed extensively worldwide. However, how worker bees of Ac and Am are adapted to their colony organization remains to be uncovered. Here, we found that the expression level of gene encoding antennae-specific proteins 1 (ASP1, a key regulator in recognizing queen mandibular pheromone) was positively correlated with the colony sizes in both bee species, and the expression level in Am was higher than that in Ac, suggesting that ASP1 may play an important role in maintaining colony homeostasis. Using competitive binding assay, molecular docking, and site-directed mutagenesis, we then confirmed the good binding affinities of both Ac-ASP1 and Am-ASP1 to methyl p-hydroxy benzoate (HOB), and Val115 was the key amino acid. However, the affinity of Am-ASP1 was stronger than that of Ac-ASP1. EAG analysis further demonstrated that antennae of Am worker bees had faster depolarization and repolarization in response to HOB stimulation. Taken together, these findings indicate that the differences in expression levels and binding dy-namics allow ASP1 recognizing HOB to potentially serve as a specific regulator of colony organization in Ac and Am.

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