Development and Application of a Visual Duck Meat Detection Strategy for Molecular Diagnosis of Duck-Derived Components
文献类型： 外文期刊
作者： Chen, Xiaoyun 1 ; Yu, Huiru 1 ; Ji, Yi 1 ; Wei, Wei 1 ; Peng, Cheng 1 ; Wang, Xiaofu 1 ; Xu, Xiaoli 1 ; Sun, Meihao 2 ; Xu, Junfeng 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
2.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321001, Zhejiang, Peoples R China
关键词： RPA; PCR; colloidal gold immunoassay strips
期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）
ISSN：
年卷期： 2022 年 11 卷 13 期
页码：
收录情况： SCI
摘要： To make meat adulteration detection systems faster, simpler and more efficient, we established a duck-derived meat rapid detection Recombinase Polymerase Amplification (dRPA) method by using interleukin 2 (IL-2) from nuclear genomic DNA as the target gene to design specific primers. We tested the dRPA detection system by comparing its sensitivity and specificity using real-time fluorescent PCR technology. By adjusting the ratio of reagents, this method shortens the time of DNA extraction and visualizes results in combination with colloidal gold immunoassay strips. Our results demonstrate that this dRPA method could specifically detect duck-derived components with a sensitivity of up to 23 copies/mu L and the accuracy of the results is consistent with real-time fluorescent PCR. Additionally, dRPA can detect at least 1% of the duck meat content by mixing beef and mutton with duck meat in different proportions, which was verified by spot-check market samples. These results can be visualized with colloidal gold immunoassay strips with the same accuracy as real-time fluorescent RPA. dRPA can complete detection within 30 min, which shortens existing detection time and quickly visualizes the detection results on-site. This lays the groundwork for future large-scale standardized duck origin detection.
- 相关文献
作者其他论文 更多>>
-
Combined exposure to microplastics and copper elicited size-dependent uptake and toxicity responses in red swamp crayfish ( Procambarus clarkia)
作者：Zeng, Huixin;Zhong, Yanfei;Luo, Mingzhong;Wei, Wei;Xu, Xiaoli
关键词：Microplastics; Copper; Accumulation; Combined toxicity; Antioxidant enzyme
-
A Novel Quantification Method for Gene-Edited Animal Detection Based on ddPCR
作者：Wang, Kaili;Lan, Hangzhen;Ji, Yi;Peng, Cheng;Wang, Xiaofu;Yang, Lei;Xu, Junfeng;Chen, Xiaoyun;Ji, Yi;Peng, Cheng;Wang, Xiaofu;Yang, Lei;Xu, Junfeng;Chen, Xiaoyun
关键词：gene editing; MSTN; nucleic acid detection; ddPCR
-
An Ultra-Sensitive Quarantine Pathogen On-Site Detection Based on a One-Pot Asymmetric Recombinase Polymerase Amplification and MNAzyme-Assisted Target Recycling Biosensor (OAR-MNA)
作者：Yang, Lei;Peng, Cheng;Chen, Xiaoyun;Xu, Xiaoli;Wei, Wei;Wang, Xiaofu;Xu, Junfeng;Chen, Guanwei;Yan, Jiatong;Sun, Meihao;Bo, Yongming;Fang, Xiaoxue;Wu, Jian
关键词：asymmetric RPA; bacterial fruit blotch; cucumber green mottle mosaic virus; MNAzyme; one-pot; ssDNA
-
Ubiquitination of the PpMADS2 transcription factor controls linalool production during UV-B irradiation in detached peach fruit
作者：Wei, Chunyan;Yang, Huizhen;Ye, Bingbing;Chen, Kunsong;Li, Xian;Zhang, Bo;Wei, Chunyan;Wei, Wei;Shan, Wei;Chen, Jianye;Chen, Kunsong;Deng, Zhiping;Zhang, Bo
关键词：
-
Development of a novel Cas13a/Cas12a-mediated 'one-pot' dual detection assay for genetically modified crops
作者：Ding, Lin;Wang, Xiaofu;Chen, Xiaoyun;Xu, Xiaoli;Wei, Wei;Yang, Lei;Ji, Yi;Xu, Junfeng;Peng, Cheng;Wu, Jian
关键词：Dual CRISPR; Genetically Modified (GM) detection; One-pot reaction; Recombinase polymerase amplification; (RPA); Multiplexed lateral flow strips
-
Microbiota-Gut-Brain Axis in Age-Related Neurodegenerative Diseases
作者：Nie, Tong;Wu, Qinghua;You, Li;Tang, Fang;Duan, Yanhui;Nepovimova, Eugenie;Kuca, Kamil;Wu, Qinghua;Kuca, Kamil;Wei, Wei
关键词：Neurodegenerative diseases; gut microbiota; blood-brain barrier; microbiota-gut-brain axis; therapy; dietary adaptations
-
A Novel Allelic Variant of OsAGPL2 Influences Rice Eating and Cooking Quality
作者：Dan, Yuqing;He, Yong;Peng, Ruixiao;Tian, Zhihong;Huang, Fudeng;Li, Chunshou;Song, Jiayu;Hao, Yuanyuan;Xu, Junfeng;Xu, Junfeng
关键词：rice quality;
OsAGPL2 ; white-core endosperm mutant; superior haplotype
