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Detection of Porcine Circovirus Type 3 in Serum, Semen, Oral Fluid, and Preputial Fluid Samples of Boars

文献类型： 外文期刊

作者： Qi, Song 1 ; He, Qiyun 1 ; Zhang, Zhewei 1 ; Chen, Huanchun 1 ; Gimenez-Lirola, Luis 4 ; Yuan, Fangyan 5 ; Bei, Weicheng 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, Natl Key Lab Agr Microbiol, Wuhan 430000, Peoples R China

2.Huazhong Agr Univ, Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan 430000, Peoples R China

3.Hubei Hongshan Lab, Wuhan 430000, Peoples R China

4.Iowa State Univ, Coll Vet Med, Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA

5.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430000, Peoples R China

关键词： PCV3; serum; semen; oral fluid; preputial fluid

期刊名称：VETERINARY SCIENCES （ 影响因子：2.4； ）

ISSN：

年卷期： 2023 年 10 卷 12 期

页码：

收录情况： SCI

摘要： Simple Summary Porcine circovirus type 3 (PCV3), a virus with various detrimental effects on pigs including respiratory disease, digestive disorders, congenital tremors, rectal prolapse, reproductive problems, and multisystemic inflammation, is widespread in pig populations worldwide. This research aims to examine the transmission, risk factors, mode of transmission, and epidemiology of PCV3 originating from boars to pig farms. The investigation involves analyzing fresh semen, as well as matched serum, oral fluid, and preputial hydrocele samples collected from 28 boars on a large-scale pig farm located in Guangxi, China. The study reveals that the PCV3 DNA is commonly found in oral fluid (64.28%) and preputial fluid (46.4%), but rarely detected in serum (3.57%), with no detection in semen. This is the first documented case of PCV3 detection in preputial fluid from boars. The results indicate that PCV3 spreads among boars on pig farms and exhibits epidemic characteristics. The paragraph provides insights for monitoring, preventing, and controlling PCV3 on pig farms. It highlights the importance of manually removing preputial fluids before semen collection to reduce the risk of contamination in both the semen and the collection vessel. Additionally, it offers other recommendations for preventing and controlling PCV3 in pig farms.Abstract Porcine circovirus type 3 (PCV3) is commonly associated with clinical symptoms such as porcine dermatitis and nephropathy syndrome (PDNS)-like lesions, respiratory signs, and reproductive disorders. This study aimed to investigate the epidemiology of PCV3 in a boar stud. The objectives were to detect PCV3 in semen, as well as matched serum, oral fluid, and preputial fluid samples from adult boars using quantitative polymerase chain reaction (qPCR), analyze PCV3-IgG antibody data, and genetically characterize a positive sample. A total of 112 samples from 28 boars were collected from a large-scale pig farm in Guangxi, China. The qPCR results showed that the PCV3 DNA was not detected in semen, with a positive rate of 0% (0/28), while it was detected in serum (3.57%-1/28), oral fluid (64.28%-18/28), and preputial fluid (46.4%-13/28). The seropositivity rate of PCV3-IgG in serum was 82.14% (23/28) according to the indirect enzyme-linked immunosorbent serologic assay (ELISA) results. Phylogenetic analysis revealed that one of the PCV3 isolates belonged to the PCV3c clades. This is the first report of PCV3 detection in preputial fluid from boars. The results suggest that PCV3 is transmitted among boars on pig farms and exhibits epidemic characteristics.

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