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Preliminary fractionation and derivatives changes of myoglobin from tilapia (O. niloticus female xO. aureus male) dark muscle with ammonium sulfate

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文献类型：外文期刊

作者： Cen, Jianwei ¹ ; Hao, Shuxian ¹ ; Li, Laihao ¹ ; Yang, Xianqing ¹ ; Hui, Huang ¹ ; Hong, Shi ¹ ; Wei, Ya ¹ ; He, Junyan ² ;

作者机构： 1.Chinese Acad Fishery Sci, S China Sea Fishery Res Inst, Guangzhou 510300, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, Agr Machinery Res Inst, Zhanjiang 524091, Peoples R China

关键词： preliminary fractionation;tilapia;myoglobin;ammonium sulfate

期刊名称：BIOTECHNOLOGY, CHEMICAL AND MATERIALS ENGINEERING, PTS 1-3

ISSN： 1022-6680

年卷期： 2012 年 393-395 卷

页码：

收录情况： SCI

摘要： Ammonium sulfate fractionation was implicated as one of essential steps to purified Myoglobin (Mb). We adopted SDS-PAGE analysis and absorption spectra scanning to demonstrate the effect of ammonium sulfate on Mb and its derivatives. The results shown that protein with the molecular weight above 37.8kDa were dominated in myoglobin extract of tilapia, which can be precipitated with ammonium sulfate. Mb molecular weight of tilapia is about 15.8 kDa, which can be collected by the treatment of ammonium sulfate with saturation between 60%-70% to remove unwanted protein. The absorption spectra show that Mb derivatives could be transferred to met-myoglobin after preliminary fractionating with ammonium sulfate.

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