文献类型： 外文期刊

作者： Xu, Hua ¹ ; He, Xinlong ³ ; Ahn, Juhee ¹ ;

作者机构： 1.Kangwon Natl Univ, Inst Biosci & Biotechnol, Chunchon, Gangwon, South Korea

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China

3.Kangwon Natl Univ, Dept Biomat Engn, Chunchon, Gangwon, South Korea

关键词： Antibiotic resistance;Staphylococcus aureus;Salmonella Typhimurium;biofilm formation;cross-protection

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 57 期

页码：

收录情况： SCI

摘要： This study was designed to evaluate the antibiotic susceptibilities, genotypic characteristics and biofilm formation abilities of antibiotic-sensitive Staphylococcus aureus KACC 13236 (SA(S)), multiple antibiotic-resistant S. aureus CCARM 3080 (SA(R)), antibiotic-sensitive Salmonella Typhimurium KCCM 40253 (ST(S)) and multiple antibiotic-resistant Salmonella Typhimurium CCARM 8009 (ST(R)) cultured in various levels of pHs (4.0, 5.5 and 7.3) and temperatures (4, 20 and 37 degrees C). The SA(S) biofilm cells were highly resistant to ampicillin, cephalothin, oxacillin and piperacillin (MIC(90) > 256 mu g/ml) compared with the SA(S) planktonic cells. The ST(R) strain was less susceptible to cephalothin, oxacillin and piperacillin than the ST(S). The SA(R) strain was positive for the staphylococcal enterotoxin genes (sec, seg, sei, sel, sem, sen and seo). The highest biofilm formation index (BFI) was 2.07 for the SA(R) biofilm cells cultured at 37 degrees C and pH 5.5. The antibiotic-resistant pathogens showed multiple antibiotic resistance, genotypic heterogeneity and cross-protective responses to low pH in the form of biofilms. This study provides useful information for the comparison of antibiotic resistance patterns and biofilm formation abilities between antibiotic-sensitive and resistant pathogens in planktonic and biofilm cells.