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Genetic identification of two species of Pleuronichthys by DNA barcoding

文献类型: 外文期刊

作者: Zhang Hui 1 ; Zhang Yan 2 ; Gao Tianxiang 1 ; Li Pengfei 3 ; Xu Hanxiang 3 ;

作者机构: 1.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

3.Marine Fisheries Res Inst Zhejiang Prov, Zhoushan 316100, Peoples R China

关键词: Pleuronichthys;DNA barcoding;COI;CR;Cyt b

期刊名称:CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY ( 2019影响因子:1.068; 五年影响因子:0.983 )

ISSN: 0254-4059

年卷期: 2011 年 29 卷 5 期

页码:

收录情况: SCI

摘要: DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding (cytochrome c oxidase subunit I (COI)), the mtDNA control region and cytochrome b. The results reveal that: 1) intraspecific variation in the DNA barcode is much less than interspecific variation; 2) the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3) COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.

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