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Molecular cloning and expression analysis of a F-type lectin gene from Japanese sea perch (Lateolabrax japonicus)

文献类型： 外文期刊

作者： Qiu, Lihua 1 ; Lin, Liansheng 2 ; Yang, Keng 1 ; Zhang, Hanhua 1 ; Li, Jianzhu 1 ; Zou, Falin 1 ; Jiang, Shigui 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Biotechnol & Aquiculture Lab, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Beijing 100039, Peoples R China

关键词： F-type lectin;Cloning;Expression;Japanese sea perch

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2011 年 38 卷 6 期

页码：

收录情况： SCI

摘要： The techniques of homology cloning and anchored PCR were used to clone the fucose-binding lectin (F-type lectin) gene from Japanese sea perch (Lateolabrax Japonicus). The full-length cDNA of sea perch F-lectin (JspFL) contained a 5' untranslated region (UTR) of 39 bp, an ORF of 933 bp encoding a polypeptide of 310 amino acids with an estimated molecular mass of 10.82 kDa and a 3' UTR of 332 bp. The searches for nucleotides and protein sequence similarities with BLAST analysis indicated that the deduced amino acid sequence of JspFL was homological to the Fucose-binding lectin in other fish species. In the JspFL deduced amino acid sequence, two tandem domains that exhibit the eel carbohydrate-recognition sequence motif were found. The temporal expressions of gene in the different tissues were measured by real-time PCR. And the mRNA expressions of the gene were constitutively expressed in tissues including spleen, head-kidney, liver, gill, and heart. The JspFL expression in spleen was different during the stimulated time point, 2 h later the expression level became up-regulated, and 6 h later the expression level became down-regulated. The result indicated that JspFL was constitutive and inducible expressed and could play a critical role in the host-pathogen interaction.

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