Nile tilapia TBK1 interacts with STING and TRAF3 and is involved in the IFN-β pathway in the immune response
文献类型： 外文期刊
作者： Zheng, Qiuyue 1 ; Gao, Fengying 1 ; Liu, Zhigang 1 ; Sun, Chengfei 1 ; Dong, Junjian 1 ; Zhang, Hetong 1 ; Ke, Xiaoli 1 ; Lu, Maixin 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fishery Resource Applicat &, Minist Agr, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, 1 Xing Yu Rd, Guangzhou 510380, Guangdong, Peoples R China
关键词： Nile tilapia ( Oreochromis niloticus ); TBK1; Immune response; Expression profile
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 1050-4648
年卷期： 2023 年 142 卷
页码：
收录情况： SCI
摘要： Nile tilapia (Oreochromis niloticus) occupies an important position in the culture of economic fish in China. However, the high mortality caused by streptococcal disease has had a significant impact on the tilapia farming industry. Therefore, it is necessary to clarify the immune mechanism of tilapia in response to Streptococcus agalactiae. As a hub in the natural immune signaling pathway, the junction molecule can help the organism defend against and clear pathogens and is crucial in the signaling pathway. In this study, the cDNA sequence of Nile tilapia TBK1 was cloned, and the expression profile was examined in normal fish and challenged fish. The cDNA sequence of the TBK1 gene was 3378 bp, and its open reading frame (ORF) was 2172 bp, encoding 723 amino acids. The deduced TBK1 protein contained an S_TKc domain, a coiled coil domain and a ubiquitin-like domain (ULD). TBK1 had the highest homology with zebra mbuna (Maylandia zebra) and Lake Malawi cichlid fish (Astatotilapia calliptera), both at 97.59%. In the phylogenetic tree, TBK1 forms a large branch with other scleractinian fish. TBK1 expression was highest in the brain and lowest in the liver. LPS, Poly I:C, and S. agalactiae challenge resulted in significant changes in TBK1 expression in the tissues examined. The subcellular localization showed that TBK1-GFP was distributed in the cytoplasm and could significantly increase IFN-beta activation. Pull-down results showed that there was an interaction between TBK1 and TRAF3 and an interaction between STING protein and TBK1 protein. The above results provide a basis for further investigation into the mechanism of TBK1 involvement in the signaling pathway.
- 相关文献
作者其他论文 更多>>
-
A multiple-population study reveals genetic variation and genes associated with resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)
作者：Wang, Zhang;Liu, Zhigang;Huang, Shaowei;Zhu, Weijuan;Cao, Jianmeng;Wang, Miao;Yi, Mengmeng;Lu, Maixin;Ke, Xiaoli;Liu, Zhigang;Huang, Shaowei;Zhu, Weijuan;Lu, Maixin;Ke, Xiaoli
关键词：Tilapia; Streptococcus agalactiae; GWAS; Selective sweep analysis; SNP marker
-
Chromosome-level genome assembly of Jaguar guapote (Parachromis manguensis) by massive parallel sequencing
作者：Cao, Jianmeng;Chen, Huizi;Liu, Zhigang;Tong, Yannan;Xiao, Zhigang
关键词：
-
Integrated gut microbiome and serum metabolomics analysis to reveal seawater adaptation strategy in Chinese sturgeon
作者：Leng, Xiaoqian;Luo, Jiang;Zhong, Jia;Wu, Jinping;Qiao, Xinmei;Xiong, Wei;Liu, Zhigang;Du, Hao
关键词：Seawater acclimation; 16S rRNA sequencing; Correlation analysis; Carbohydrate metabolism
-
Comparative analysis of the intestinal flora of Siniperca chuatsi at different growth stages under three feeding practices
作者：Yan, Yunyun;Chen, Xiao;Dong, Junjian;Gao, Fengying;Zhang, Hetong;Ye, Xing;Sun, Chengfei;Yan, Yunyun;Wu, Chengbin;Wang, Fubao
关键词：Compound feed; Live bait; Intestinal flora; 16S rRNA sequencing; Siniperca chuatsi
-
Nile tilapia P38 MAPK interacts with Tak1 and is involved in the immune response to Streptococcus agalactiae, PolyI:C, and LPS stimulation
作者：Zheng, Qiuyue;Liu, Zhigang;Sun, Chengfei;Dong, Junjian;Zhang, Hetong;Ke, Xiaoli;Gao, Fengying;Lu, Maixin;Zheng, Qiuyue
关键词：Nile tilapia ( Oreochromis niloticus ); P38 MAPK; Immune response; Expression profile
-
Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Mechanisms Involved in the Adaptations of Mandarin Fish (Siniperca chuatsi) to Compound Feed
作者：Yan, Yunyun;Zhang, Yuan;Dong, Junjian;Zhang, Hetong;Gao, Fengying;Ye, Xing;Sun, Chengfei;Yan, Yunyun;Wu, Chengbin;Wang, Fubao
关键词：
Siniperca chuatsi ; compound feed; transcriptome; metabolome; integrated analysis -
Clock gene NR1D1 mediated inflammatory response after Streptococcus agalactiae challenge in Nile Tilapia (Oreochromis niloticus) brain
作者：Wang, Zhang;Liu, Zhigang;Zhu, Weijuan;Zhang, Meiyan;Huang, Shaowei;Cao, Jianmeng;Wang, Miao;Yi, Mengmeng;Gao, Fengying;Lu, Maixin;Ke, Xiaoli;Liu, Zhigang;Zhu, Weijuan;Zhang, Meiyan;Huang, Shaowei;Gao, Fengying;Lu, Maixin;Ke, Xiaoli
关键词：S. agalactiae; Neuronal injury; NF-kappa B; Astrocytes inflammatory response
