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Stable and efficient expression of human brain-derived neurotrophic factor in tobacco chloroplasts

文献类型： 外文期刊

作者： Wang, Yunpeng 1 ; Xu, Nuo 2 ; Fan, Jieying 1 ; Wei, Zhengyi 1 ; Xin, Wen 3 ; Xing, Shaochen 1 ;

作者机构： 1.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun, Jilin, Peoples R China

2.Wenzhou Univ, Coll Life & Environm Sci, Wenzhou, Zhejiang, Peoples R China

3.Beijing TransGen Biotech Co Ltd, Beijing, Peoples R China

关键词： Tobacco; Chloroplast; Genetic transformation; Brain-derived neurotrophic factor; Green fluorescent protein

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.742； 五年影响因子：2.702 ）

ISSN： 0301-4851

年卷期：

页码：

收录情况： SCI

摘要： Background Brain-derived neurotrophic factor (BDNF) is an intensively studied neurotrophin that promotes various physiological processes, such as acceleration of cell proliferation and differentiation, and is, therefore widely used in clinical applications. Methods and Results In this study, an expression vector with a codon-optimized hBDNF gene was constructed and transferred into chloroplasts of tobacco by gene-gun. After three or four rounds of selection with optimal spectinomycin concentration, hBDNF was integrated into the chloroplast genome of homoplastomic plants, as confirmed by PCR and Southern hybridization. ELISA indicated that hBDNF fused with GFP represented approximately 15.72% +/- 0.33% of total soluble protein in the leaves of transplastomic plants. Moreover, the chloroplast-derived hBDNF displayed biological activity similar to the commercial product. Conclusions This is the first case report of hBDNF expression by chloroplast transformation in the plant model, providing an additional pathway for the production of chloroplast-expressed therapeutic proteins.

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