您好,欢迎访问江苏省农业科学院 机构知识库!

Yeast Synthesis and Herbicidal Activity Evaluation of Aspterric Acid

文献类型: 外文期刊

作者: Zhou, Zhenzhen 1 ; Zhang, Yuting 1 ; Wu, Qi 1 ; Hou, Xiaodong 1 ; Zhang, Baolong 1 ;

作者机构: 1.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Nanjing 210014, Jiangsu, Peoples R China

3.Zhongshan Biol Breeding Lab, Nanjing 210014, Jiangsu, Peoples R China

关键词: aspterric acid; Saccharomyces cerevisiae; biosynthesis-related gene cluster; sesquiterpenecyclase; herbicidal activity

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:6.2; 五年影响因子:6.4 )

ISSN: 0021-8561

年卷期: 2024 年 72 卷 46 期

页码:

收录情况: SCI

摘要: Aspterric acid (AA) is a novel natural product herbicide that targets dihydroxyacid dehydratase in plants. In this study, we introduced two distinct AA biosynthesis-related gene clusters into Saccharomyces cerevisiae and screened the core biosynthetic enzyme, sesquiterpene cyclase, from various fungi. The combination of sesquiterpene cyclase from Aspergillus taichungensis IBT 19404 and two cytochrome P450s from Penicillium brasilianum resulted in the optimal AA synthesis efficiency in yeast, with the highest titer of 33.21 mg/L achieved by optimizing fermentation conditions in shake flasks. Moreover, the herbicidal effects of AA on weed germination and growth were evaluated. Notably, AA strongly inhibited the germination of Amaranthus tricolor, Portulaca oleracea, Bidens pilosa, Lolium perenne, and Leptochloa chinensis. Furthermore, AA could also inhibit the shoot and root growth of weeds with a superior inhibitory effect on roots relative to shoots. Our work not only provides a sustainable method for the biosynthesis of AA in yeast but also paves the way for the application of AA as a preemergence herbicide.

  • 相关文献
作者其他论文 更多>>