文献类型： 外文期刊

作者： Li, Jidong ¹ ; Chen, Can ¹ ; Gao, Le ¹ ; Wang, Lingling ¹ ; Wang, Wei ¹ ; Zhang, Jinhua ¹ ; Gong, Zhenxing ¹ ; Wang, Jiandong ² ; Guo, Yanan ² ;

作者机构： 1.Ningxia Univ, Sch Agr, Yinchuan 750021, Ningxia, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan 750002, Ningxia, Peoples R China

关键词： Sheep; Mycoplasma ovipneumoniae; Histopathological analysis; Immunohistochemistry; Cytokine

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.9 ）

ISSN：

年卷期： 2023 年 19 卷 1 期

页码：

收录情况： SCI

摘要： Background Mycoplasma ovipneumoniae (M. ovipneumoniae) is one of the main pathogens of sheep pneumonia, causing a series of clinical symptoms, such as depression, anorexia, hyperthermia, cough, dyspnea, and tract secretions. In recent years, the prevalence of M. ovipneumoniae pneumonia has become increasingly serious in sheep farms in Ningxia, China, leading to the death of sheep, and causing significant economic losses. In this study, the pathological organs infected by M. ovipneumoniae were collected to observe histopathological change, to determine the tissue localization of M. ovipneumoniae, and to analyze the cytokine changes, which lays a basis for the diagnosis and pathogenesis of M. ovipneumoniae disease.Results In this study, M. ovipneumoniae was detected in 97 of 105 samples collected from 13 large-scale sheep farms for nucleic acid by PCR. One representative isolate per farm was isolated from 13 farms. The lesions caused by M. ovipneumoniae were mainly in the trachea, bronchus, and lung, including necrosis of tracheal mucosal epithelial cells, disintegration of some epithelial cells, edema of mucosal lamina propria, with inflammatory cell infiltration, cytoplasmic vacuolization of epithelial cells of bronchial mucosa, massive infiltration of inflammatory cells in the alveolar space of lung, necrosis and hyperplasia of alveolar epithelial cells. Immunohistochemical analysis showed that the proportion of M. ovipneumoniae positive area in the lung was the largest, followed by that in the bronchus and trachea. Compared to healthy animals, diseased animals exhibited up-regulated gene expression levels of IL-1 beta, IL-6, and NF-kappa B in the trachea, bronchus, and lungs. In contrast, the expression of IL-10, IL-12, and IFN-gamma was primarily limited to the trachea and bronchus. The expression of IL-1 beta showed differential patterns across different lung regions, with variations observed among lung lobes. Additionally, other cytokines consistently showed significant up-regulation specifically in the bronchus.Conclusions M. ovipneumoniae is primarily found in the lungs of infected individuals. NF-kappa B, an essential transcription factor, is involved in the regulation of IL-1 beta transcription. IL-12 may enhance the cytotoxic function of natural killer cells during M. ovipneumoniae infection. Those findings demonstrate the distinct expression profiles of cytokines in various anatomical sites throughout disease progression, suggesting the potential role of bronchial tissue as a major site of immune response.