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QTL mapping and candidate gene mining of flag leaf size traits in Japonica rice based on linkage mapping and genome-wide association study

文献类型： 外文期刊

作者： Wang, Jiangxu 1 ; Wang, Tao 1 ; Wang, Qi 1 ; Tang, Xiaodong 1 ; Ren, Yang 1 ; Zheng, Haiyan 1 ; Liu, Kai 1 ; Yang, Luomia 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Inst Crops Tillage & Cultivat, Harbin 150030, Peoples R China

2.Northeast Agr Univ, Key Lab Germplasm Enhancement Physiol & Ecol Food, Minist Educ, Harbin 150030, Peoples R China

关键词： Japonica rice; QTLs; Flag leaf; Linkage mapping; Genome-wide association study

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期：

页码：

收录情况： SCI

摘要： Background As one of the most important factors of the japonica rice plant, leaf shape affects the photosynthesis and carbohydrate accumulation directly. Mining and using new leaf shape related genes/QTLs can further enrich the theory of molecular breeding and accelerate the breeding process of japonica rice. Methods In the present study, 2 RILs and a natural population with 295 japonica rice varieties were used to map QTLs for flag leaf length (FL), flag leaf width (FW) and flag leaf area (FLA) by linkage analysis and genome-wide association study (GWAS) throughout 2 years. Results A total of 64 QTLs were detected by 2 ways, and pleiotropic QTLs qFL2 (Chr2_33,332,579) and qFL10 (Chr10_10,107,835; Chr10_10,230,100) consisted of overlapping QTLs mapped by linkage analysis and GWAS throughout the 2 years were identified. Conclusions The candidate genes LOC_Os02g54254, LOC_Os02g54550, LOC_Os10g20160, LOC_Os10g20240, LOC_Os10g20260 were obtained, filtered by linkage disequilibrium (LD), and haplotype analysis. LOC_Os10g20160 (SD-RLK-45) showed outstanding characteristics in quantitative real-time PCR (qRT-PCR) analysis in leaf development period, belongs to S-domain receptor-like protein kinases gene and probably to be a main gene regulating flag leaf width of japonica rice. The results of this study provide valuable resources for mining the main genes/QTLs of japonica rice leaf development and molecular breeding of japonica rice ideal leaf shape.

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