A Rationally Designed H5 Hemagglutinin Subunit Vaccine Provides Broad-Spectrum Protection against Various H5Nx Highly Pathogenic Avian Influenza Viruses in Chickens
文献类型： 外文期刊
作者： Zhang, Xuxiao 1 ; Zhang, Fushou 1 ; Chen, Ning 1 ; Cui, Xiaoping 3 ; Guo, Xiaoqin 1 ; Sun, Zhi 1 ; Guo, Pengju 1 ; Liao, Ming 2 ; Li, Xin 1 ;
作者机构： 1.Boehringer Ingelheim Vetmed China Co Ltd, Taizhou 225300, Peoples R China
2.South China Agr Univ, Coll Vet Med, Natl & Reg Joint Engn Lab Medicament Zoonosis Prev, Guangzhou 510642, Peoples R China
3.Boehringer Ingelheim Anim Hlth USA Inc, 3239 Satellite Blvd, Duluth, GA 30096 USA
4.Guangdong Acad Agr Sci, Minist Agr & Rural Affairs, Inst Anim Hlth, Key Lab Prevent & Control Avian Influenza & Other, Guangzhou 510640, Peoples R China
5.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510550, Peoples R China
关键词： avian influenza virus; H5 subtype; subunit vaccine; broad protection
期刊名称：VACCINES （ 影响因子：3.4； 五年影响因子：3.7 ）
ISSN：
年卷期： 2024 年 12 卷 8 期
页码：
收录情况： SCI
摘要： The evolution of the H5 highly pathogenic avian influenza (HPAI) viruses has led to the emergence of distinct groups with genetically similar clusters of hemagglutinin (HA) sequences. In this study, a consensus H5 HA sequence was cloned into the baculovirus expression system. The HA protein was expressed in baculovirus-infected insect cells and utilized as the antigen for the production of an oil emulsion-based H5 avian influenza vaccine (rBacH5Con5Mut). Twenty-one-day-old SPF chickens were immunized with this vaccine and then challenged at 21 days post-vaccination with clade 2.3.2.1, clade 2.3.4.4, and clade 7.2 of H5 HPAI viruses. The sera of vaccinated chickens exhibited high hemagglutination inhibition (HI) titers against the rBacH5 vaccine antigen, while lower HI titers were observed against the different challenge virus H5 hemagglutinins. Furthermore, the rBacH5Con5Mut vaccine provided 100% protection from mortality and clinical signs. Virus isolation results showed that oropharyngeal and cloacal shedding was prevented in 100% of the vaccinated chickens when challenged with clade 2.3.2.1 and clade 2.3.4.4 H5 viruses. When the rBacH5Con5Mut vaccine candidate was administrated at one day of age, 100% protection was demonstrated against the challenge of clade 2.3.4.4 virus at three weeks of age, indicating the potential of this vaccine for hatchery vaccination. Overall, A single immunization of rBacH5Con5Mut vaccine candidate with a consensus HA antigen can protect chickens against different clades of H5 HPAI viruses throughout the rearing period of broiler chickens without a boost, thus fulfilling the criteria for an efficacious broad-spectrum H5 avian influenza vaccine.
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