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WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and indole-3-acetic acid-conjugating genes and by inhibiting ammonium efflux in the root elongation zone

文献类型： 外文期刊

作者： Di, Dong-Wei 1 ; Sun, Li 1 ; Wang, Meng 1 ; Wu, Jingjing 1 ; Kronzucker, Herbert J. 4 ; Fang, Shuang 6 ; Chu, Jinfang; 1 ;

作者机构： 1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China

2.Nanjing Agr Univ, Cytogenet Inst, State Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Nanjing 210095, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Jiangsu, Peoples R China

4.Univ Melbourne, Sch BioSci, Parkville, Vic 3010, Australia

5.Univ British Columbia, Fac Land & Food Syst, Vancouver, BC V6T IZ4, Canada

6.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, Beijing 100049, Peoples R China

关键词： free IAA; NH4+ efflux; NH4+ toxicity; protein N-glycosylation; root growth inhibition; WRKY46

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.152； 五年影响因子：10.476 ）

ISSN： 0028-646X

年卷期：

页码：

收录情况： SCI

摘要： Ammonium (NH4+) is toxic to root growth in most plants, even at moderate concentrations. Transcriptional regulation is one of the most important mechanisms in the response of plants to NH4+ toxicity, but the nature of the involvement of transcription factors (TFs) in this regulation remains unclear. Here, RNA-seq analysis was performed on Arabidopsis roots to screen for ammonium-responsive TFs. WRKY46, the member of the WRKY transcription factor family most responsive to NH4+, was selected. We defined the role of WRKY46 using mutation and overexpression assays, and characterized the regulation of NUDX9 and indole-3-acetic acid (IAA)-conjugating genes by WRKY46 via yeast one-hybrid and electrophoretic mobility shift assays and chromatin immunoprecipitation-quantitative real-time polymerase chain reaction (ChIP-qPCR). Knockout of WRKY46 increased, while overexpression of WRKY46 decreased, NH4+-suppression of the primary root. WRKY46 is shown to directly bind to the promoters of the NUDX9 and IAA-conjugating genes (GH3.1, GH3.6, UGT75D1, UGT84B2) and to inhibit their transcription, thus positively regulating free IAA content and stabilizing protein N-glycosylation, leading to an inhibition of NH4+ efflux in the root elongation zone (EZ). We identify TF involvement in the regulation of NH4+ efflux in the EZ, and show that WRKY46 inhibits NH4+ efflux by negative regulation of NUDX9 and IAA-conjugating genes.

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