您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

CRISPR/Cas9-mediated knockout of Bsr-d1 enhances the blast resistance of rice in Northeast China

文献类型： 外文期刊

作者： Zhang, Ying 1 ; Lin, Xiu-Feng 1 ; Li, Li 3 ; Piao, Ri-Hua 2 ; Wu, Songquan 4 ; Song, Anqi 1 ; Gao, Mengmeng 1 ; Jin, Yong-Mei 1 ;

作者机构： 1.Jilin Acad Agr Sci, Res Inst Agr Biotechnol, Jilin Prov Key Lab Agr Biotechnol, Changchun 130033, Peoples R China

2.Jilin Acad Agr Sci, Rice Res Inst, Gongzhuling 136100, Peoples R China

3.Jilin Acad Agr Sci, Inst Plant Protect, Jilin Key Lab Agr Microbiol,Minist Agr & Rural Aff, Key Lab Integrated Pest Management Crops Northeast, Gongzhuling 136100, Peoples R China

4.Yanbian Univ, Coll Agr Sci, Yanji 133000, Peoples R China

关键词： Bsr-d1; Rice blast; Japonica rice; CRISPR/Cas9

期刊名称：PLANT CELL REPORTS （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0721-7714

年卷期： 2024 年 43 卷 4 期

页码：

收录情况： SCI

摘要： Rice blast is a global disease that has a significant negative impact on rice yield and quality. Due to the complexity and variability of the physiological races of rice blast, controlling rice blast is challenging in agricultural production. Bsr-d1, a negative transcription factor that confers broad-spectrum resistance to rice blast, was identified in the indica rice cultivar Digu; however, its biological function in japonica rice varieties is still unclear. In this study, we analyzed the blast resistance allele of Bsr-d1 in a total of 256 japonica rice varieties from Jilin Province in Northeast China and found that this allele was not present in these varieties. Therefore, we generated Bsr-d1 knockout mutants via the CRISPR/Cas9 system using the japonica rice variety Jigeng88 (JG88) as a recipient variety. Compared with those of the wild-type JG88, the homozygous Bsr-d1 mutant lines KO#1 and KO#2 showed enhanced leaf blast resistance at the seedling stage to several Magnaporthe oryzae (M. oryzae) races collected from Jilin Province in Northeast China. Physiological and biochemical indices revealed that the homozygous mutant lines produced more hydrogen peroxide than did JG88 plants when infected with M. oryzae. Comparative RNA-seq revealed that the DEGs were mainly involved in the synthesis of amide compounds, zinc finger proteins, transmembrane transporters, etc. In summary, our results indicate that the development of Bsr-d1 knockout mutants through CRISPR/Cas9 can enhance the broad-spectrum resistance of rice in Northeast China to rice blast. This study not only provides a theoretical basis for disease resistance breeding involving the Bsr-d1 gene in Northeast China, but also provides new germplasm resources for disease-resistance rice breeding.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台