文献类型： 外文期刊
作者： Wang, Yunpeng 1 ; Fan, Jieying 1 ; Wei, Zhengyi 1 ; Xing, Shaochen 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun 130033, Peoples R China
2.Guangxi Acad Agr Sci, Maize Res Inst, Nanning 530007, Peoples R China
关键词： EGF; Chloroplast transformation; Tobacco; GFP; Molecular farming
期刊名称：BMC BIOTECHNOLOGY （ 2022影响因子：3.5； 五年影响因子：3.5 ）
年卷期： 2023 年 23 卷 1 期
收录情况： SCI
摘要： Background Chloroplast transformation is a robust technology for the expression of recombinant proteins. Various types of pharmaceutical proteins including growth factors have been reported in chloroplasts via chloroplast transformation approach at high expression levels. However, high expression of epidermal growth factor (EGF) in chloroplasts with the technology is still unavailable.Results The present work explored the high-level expression of recombinant EGF, a protein widely applied in many clinical therapies, in tobacco chloroplasts. In this work, homoplastic transgenic plants expressing fusion protein GFP-EGF, which was composed of GFP and EGF via a linker, were generated. The expression of GFP-EGF was confirmed by the combination of green fluorescent observation and Western blotting. The achieved accumulation of the recom-binant fusion GFP-EGF was 10.21 +/- 0.27% of total soluble proteins (1.57 +/- 0.05 g kg(-1) of fresh leaf). The chloroplast-derived GFP-EGF was capable of increasing the cell viability of the NSLC cell line A549 and enhancing the phospho-rylation level of the EGF receptor in the A549 cells.Conclusion The expression of recombinant EGF in tobacco chloroplasts via chloroplast transformation method was achieved at considerable accumulation level. The attempt gives a good example for the application of chloroplast transformation technology in recombinant pharmaceutical protein production.
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