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Viral Decoys: The Only Two Herpesviruses Infecting Invertebrates Evolved Different Transcriptional Strategies to Deflect Post-Transcriptional Editing

文献类型： 外文期刊

作者： Bai, Chang-Ming 1 ; Rosani, Umberto 3 ; Zhang, Xiang 1 ; Xin, Lu-Sheng 1 ; Bortoletto, Enrico 4 ; Wegner, K. Mathia 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Yellow Sea Fisheries Res Inst, Key Lab Maricultural Organism Dis Control,Minist, Qingdao 266237, Peoples R China

2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China

3.Alfred Wegener Inst Helmholtz Ctr Polar & Marine, Coastal Ecol Sect, Warden Sea Stn, D-25992 List Auf Sylt, Germany

4.Univ Padua, Dept Biol, I-35121 Padua, Italy

5.Tianjin Agr Univ, Coll Fisheries, Tianjin 300380, Peoples R China

关键词： PacBio SMRT; long-read sequencing; malacoherpesvirus; OsHV-1; HaHV-1; antisense transcription; ADAR editing; host defenses

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN：

年卷期： 2021 年 13 卷 10 期

页码：

收录情况： SCI

摘要： The highly versatile group of Herpesviruses cause disease in a wide range of hosts. In invertebrates, only two herpesviruses are known: the malacoherpesviruses HaHV-1 and OsHV-1 infecting gastropods and bivalves, respectively. To understand viral transcript architecture and diversity we first reconstructed full-length viral genomes of HaHV-1 infecting Haliotis diversicolor supertexta and OsHV-1 infecting Scapharca broughtonii by DNA-seq. We then used RNA-seq over the time-course of experimental infections to establish viral transcriptional dynamics, followed by PacBio long-read sequencing of full-length transcripts to untangle viral transcript architectures at two selected time points. Despite similarities in genome structure, in the number of genes and in the diverse transcriptomic architectures, we measured a ten-fold higher transcript variability in HaHV-1, with more extended antisense gene transcription. Transcriptional dynamics also appeared different, both in timing and expression trends. Both viruses were heavily affected by post-transcriptional modifications performed by ADAR1 affecting sense-antisense gene pairs forming dsRNAs. However, OsHV-1 concentrated these modifications in a few genomic hotspots, whereas HaHV-1 diluted ADAR1 impact by elongated and polycistronic transcripts distributed over its whole genome. These transcriptional strategies might thus provide alternative potential roles for sense-antisense transcription in viral transcriptomes to evade the host's immune response in different virus-host combinations.

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