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Paired miRNA and RNA sequencing provides a first insight into molecular defense mechanisms of Scapharca broughtonii during ostreid herpesvirus-1 infection

文献类型： 外文期刊

作者： Bai, Chang-Ming 1 ; Zhang, Xiang 1 ; Venier, Paola 4 ; Gu, Li 1 ; Li, Ya-Nan 1 ; Wang, Chong-Ming 1 ; Xin, Lu-Sheng 1 ; Rosani, Umberto 4 ;

作者机构： 1.Chinese Acad Fishery Sci, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Key Lab Maricultural Organism Dis Control, Minist Agr,Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China

3.Tianjin Agr Univ, Coll Fisheries, Tianjin 300380, Peoples R China

4.Univ Padua, Dept Biol, I-35121 Padua, Italy

关键词： miRNA; OsHV-1; Blood clam; RNA-seq; Aquaculture

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 122 卷

页码：

收录情况： SCI

摘要： Ostreid herpesvirus 1 (OsHV-1) infection caused mortalities with relevant economic losses in bivalve aquaculture industry worldwide. Initially described as an oyster pathogen, OsHV-1 can infect other bivalve species, like the blood clam Scapharca broughtonii. However, at present, little is known about the molecular interactions during OsHV-1 infection in the blood clam. We produced paired miRNA and total RNA-seq data to investigate the blood clam transcriptional changes from 0 to 72 h after experimental infection with OsHV-1. High-throughput miRNA sequencing of 24 libraries revealed 580 conserved and 270 new blood clam miRNAs, whereas no genuine miRNA was identified for OsHV-1. Total 88-203 differently expressed miRNAs were identified per time point, mostly up regulated and mainly targeting metabolic pathways. Most of the blood clam mRNAs, in contrast, were down regulated up to 60 h post-injection, with the trend analysis revealing the activation of immune genes only when comparing the early and latest stage of infection. Taken together, paired short and long RNA data suggested a miRNA-mediated down-regulation of host metabolic and energetic processes as a possible antiviral strategy during early infection stages, whereas antiviral pathways appeared upregulated only at late infection.

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