Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp, nonstructural proteins p16 and p23, playing a role in virus replication
文献类型： 外文期刊
作者： Zhu, Jie 1 ; Miao, Qiuhong 1 ; Guo, Hongyuan 1 ; Tang, Aoxing 1 ; Dong, Dandan 1 ; Tang, Jingyu 1 ; Wang, Fang 3 ; Tong, Guangzhi 1 ; Liu, Guangqing 1 ;
作者机构： 1.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China
2.Wageningen Univ & Res, Lab Virol, NL-6708 PB Wageningen, Netherlands
3.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China
关键词： Rabbit hemorrhagic disease virus (RHDV); Nucleolin; Virus replication; RNA-dependent RNA polymerase (RdRp)
期刊名称：VIROLOGICA SINICA （ 影响因子：6.947； 五年影响因子：5.997 ）
ISSN： 1674-0769
年卷期： 2022 年 37 卷 1 期
页码：
收录情况： SCI
摘要： Rabbit hemorrhagic disease virus (RHDV) is a member of the Caliciviridae family and cannot be propagated in vitro, which has impeded the progress of investigating its replication mechanism. Construction of an RHDV replicon system has recently provided a platform for exploring RHDV replication in host cells. Here, aided by this replicon system and using two-step affinity purification, we purified the RHDV replicase and identified its associated host factors. We identified rabbit nucleolin (NCL) as a physical link, which mediating the interaction between other RNA-dependent RNA polymerase (RdRp)-related host proteins and the viral replicase RdRp. We found that the overexpression or knockdown of NCL significantly increased or severely impaired RHDV replication in RK-13 cells, respectively. NCL was identified to directly interact with RHDV RdRp, p16, and p23. Furthermore, NCL knockdown severely impaired the binding of RdRp to RdRp-related host factors. Collectively, these results indicate that the host protein NCL is essential for RHDV replication and acts as a physical link between viral replicase and host proteins.
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