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Degradation of Ochratoxin A by a UV-Mutated Aspergillus niger Strain

文献类型： 外文期刊

作者： Zou, Dong 1 ; Ji, Jian 1 ; Ye, Yongli 1 ; Yang, Yang 1 ; Yu, Jian 1 ; Wang, Meng 3 ; Zheng, Yi 4 ; Sun, Xiulan 1 ;

作者机构： 1.Jiangnan Univ, Synerget Innovat Ctr Food Safety & Qual Control, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

2.Xinjiang Agr Univ, Coll Food Sci & Pharm, Urumqi 830052, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & Testing Technol, Beijing 100097, Peoples R China

4.Jiangsu Agrianim Husb Vocat Coll, Key Lab High Tech Res & Dev Vet Biopharmaceut, Taizhou 214122, Peoples R China

关键词： Ochratoxin A; detoxification; Aspergillus niger; degradation products; cytotoxicity

期刊名称：TOXINS （ 影响因子：5.075； 五年影响因子：5.305 ）

ISSN：

年卷期： 2022 年 14 卷 5 期

页码：

收录情况： SCI

摘要： Ochratoxin A (OTA) is a mycotoxin that can contaminate a wide range of crops such as grains and grapes. In this study, a novel fungal mutant strain (FS-UV-21) with a high OTA degradation rate (74.5%) was obtained from Aspergillus niger irradiated with ultraviolet light (15 W for 20 min). The effect of pH, temperature, and inoculation concentration on the degradation of OTA by FS-UV-21 was investigated, and the results revealed that the detoxification effect was optimal (89.4%) at a pH of 8 and a temperature of 30 degrees C. Ultra-performance liquid chromatography-tandem mass spectrometry was used to characterize the degraded products of OTA, and the main degraded product was ochratoxin alpha. Triple quadrupole-linear ion trap-mass spectrometry combined with LightSight software was used to analyze the biotransformation pathway of OTA in FS-UV-21, to trace the degraded products, and to identify the main metabolite, P1 (C19H18ClNO6, m/z 404). After the FS-UV-21 strain was treated with OTA, the HepG2 cellular toxicity of the degradation products was significantly reduced. For the real sample, FS-UV-21 was used to remove OTA from wheat bran contaminated by mycotoxins through fermentation, resulting in the degradation of 59.8% of OTA in wheat bran. Therefore, FS-UV-21 can be applied to the degradation of OTA in agricultural products and food.

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