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Multigene manipulation of photosynthetic carbon metabolism enhances the photosynthetic capacity and biomass yield of cucumber under low-CO2 environment

文献类型： 外文期刊

作者： Chen, Zhi-Feng 1 ; Wang, Tian-Hong 2 ; Feng, Chao-Yang 1 ; Guo, Hai-Feng 1 ; Guan, Xiao-Xi 1 ; Zhang, Tian-Li 3 ; Li, Wen-Zhao 1 ; Xing, Guo-Ming 3 ; Sun, Sheng 3 ; Tan, Guo-Fei 4 ;

作者机构： 1.Coll Biol & Agr Technol, Zunyi Normal Coll, Zunyi, Peoples R China

2.Acad Agr Sci, Fruit & Vegetable Res Inst, Zunyi, Peoples R China

3.Shanxi Agr Univ, Coll Hort, Jinzhong, Peoples R China

4.Guizhou Acad Agr Sci, Inst Hort, Guiyang, Peoples R China

关键词： cucumber; carbon metabolism; low-CO2; greenhouse; multigene manipulation; highphotosynthetic efficiency

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Solar greenhouses are important in the vegetable production and widely used for the counter-season production in the world. However, the CO2 consumed by crops for photosynthesis after sunrise is not supplemented and becomes chronically deficient due to the airtight structure of solar greenhouses. Vegetable crops cannot effectively utilize light resources under low-CO2 environment, and this incapability results in reduced photosynthetic efficiency and crop yield. We used cucumber as a model plant and generated several sets of transgenic cucumber plants overexpressing individual genes, including beta-carbonic anhydrase 1 (Cs beta CA1), beta-carbonic anhydrase 4 (Cs beta CA4), and sedoheptulose-1,7-bisphosphatase (CsSBP); fructose-1,6-bisphosphate aldolase (CsFBA), and Cs beta CA1 co-expressing plants; Cs beta CA4, CsSBP, and CsFBA co-expressing plants (14SF). The results showed that the overexpression of Cs beta CA1, Cs beta CA4, and 14SF exhibited higher photosynthetic and biomass yield in transgenic cucumber plants under low-CO2 environment. Further enhancements in photosynthesis and biomass yield were observed in 14SF transgenic plants under low-CO2 environment. The net photosynthesis biomass yield and photosynthetic rate increased by 49% and 79% compared with those of the WT. However, the transgenic cucumbers of overexpressing CsFBA and CsSBP showed insignificant differences in photosynthesis and biomass yield compared with the WT under low-CO2.environment. Photosynthesis, fluorescence parameters, and enzymatic measurements indicated that Cs beta CA1, Cs beta CA4, CsSBP, and CsFBA had cumulative effects in photosynthetic carbon assimilation under low-CO2 environment. Co-expression of this four genes (Cs beta CA1, Cs beta CA4, CsSBP, and CsFBA) can increase the carboxylation activity of RuBisCO and promote the regeneration of RuBP. As a result, the 14SF transgenic plants showed a higher net photosynthetic rate and biomass yield even under low-CO(2)environment.These findings demonstrate the possibility of cultivating crops with high photosynthetic efficiency by manipulating genes involved in the photosynthetic carbon assimilation metabolic pathway.

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