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Gonadal Transcriptome Analysis and Sequence Characterization of Sex-Related Genes in Cranoglanis bouderius

文献类型： 外文期刊

作者： Wang, Dongjie 1 ; Pan, Zhengkun 1 ; Wang, Guoxia 2 ; Ye, Bin 1 ; Wang, Qiujie 1 ; Zuo, Zhiheng 1 ; Zou, Jixing 1 ; Xie, Shaolin 1 ;

作者机构： 1.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Peoples R China

2.Feed Sci South China Minist Agr, Key Lab Anim Nutr, Guangzhou 510640, Peoples R China

3.Inst Anim Sci, Guangdong Acad Agr Sci, Key Lab Anim Breeding & Nutr, Guangdong Publ Lab Anim Breeding & Nutr, Guangzhou 510640, Peoples R China

关键词： gonadal; Cranoglanis bouderius; transcriptome; molecular pathway; sex differentiation; sex-related genes

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 24 期

页码：

收录情况： SCI

摘要： In China, the Cranoglanis bouderius is classified as a national class II-protected animal. The development of C. bouderius populations has been affected by a variety of factors over the past few decades, with severe declines occurring. Considering the likelihood of continued population declines of the C. bouderius in the future, it is critical to investigate the currently unknown characteristics of gonadal differentiation and sex-related genes for C. bouderius conservation. In this study, the Illumina sequencing platform was used to sequence the gonadal transcriptome of the C. bouderius to identify the pathways and genes related to gonadal development and analyze the expression differences in the gonads. A total of 12,002 DEGs were identified, with 7220 being significantly expressed in the ovary and 4782 being significantly expressed in the testis. According to the functional enrichment results, the cell cycle, RNA transport, apoptosis, Wnt signaling pathway, p53 signaling pathway, and prolactin signaling pathway play important roles in sex development in the C. bouderius. Furthermore, the sequence characterization and evolutionary analysis revealed that AMH, DAX1, NANOS1, and AR of the C. bouderius are highly conserved. Specifically, the qRT-PCR results from various tissues showed significant differences in AMH, DAX1, NANOS1, and AR expression levels in the gonads of both sexes of C. bouderius. These analyses indicated that AMH, DAX1, NANOS1, and AR may play important roles in the differentiation and development of C. bouderius gonads. To our best knowledge, this study is the first to analyze the C. bouderius gonadal transcriptome and identify the structures of sex-related genes, laying the foundation for future research.

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