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MnFtz-f1 Is Required for Molting and Ovulation of the Oriental River Prawn Macrobrachium nipponense

文献类型： 外文期刊

作者： Yuan, Huwei 1 ; Zhang, Wenyi 2 ; Fu, Yin 3 ; Jiang, Sufei 2 ; Xiong, Yiwei 2 ; Zhai, Shuhua 2 ; Gong, Yongsheng 2 ; Qiao, Hui 2 ; Fu, Hongtuo 1 ; Wu, Yan 2 ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Jiangsu, Peoples R China

2.Chinese Acad Fishery Sci, Minist Agr, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi, Jiangsu, Peoples R China

3.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai, Peoples R China

关键词： 20E; Macrobrachium nipponense; MnFtz-f1; RNAi; molt; ovulation

期刊名称：FRONTIERS IN ENDOCRINOLOGY （ 影响因子：6.055； 五年影响因子：6.335 ）

ISSN： 1664-2392

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Molting and ovulation are the basic processes responsible for the growth and reproduction of Macrobrachium nipponense; however, the molecular mechanisms of molting and ovulation in M. nipponense are poorly understood. The present study aimed to use MnFtz-f1 as the starting point to study the molting and ovulation phenomena in M. nipponense at the molecular level. The full-length MnFtz-f1 cDNA sequence was 2,198 base pairs (bp) in length with an open reading frame of 1,899 bp encoding 632 amino acids. Quantitative real-time PCR analysis showed that MnFtz-f1 was highly expressed in the ovary at the cleavage stage and on the fifth day after hatching. In vivo administration of 20-hydroxyecdysone (20E) showed that 20E effectively inhibited the expression of the MnFtz-f1 gene, and the silencing of the MnFtz-f1 gene reduced the content of 20E in the ovary. In situ hybridization (ISH) analysis revealed the localization of MnFtz-f1 in the ovary. Silencing of MnFtz-f1 by RNA interference (RNAi) resulted in significant inhibition of the expression of the vitellogenin (Vg), Spook, and Phantom genes, thus confirming that MnFtz-f1 had a mutual regulatory relationship with Vg, Spook, and Phantom. After RNAi, the molting frequency and ovulation number of M. nipponense decreased significantly, which demonstrated that MnFtz-f1 played a pivotal role in the process of molting and ovulation.

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