文献类型： 外文期刊
作者： Xiao, Cheng 1 ; Jin, Hai Guo 1 ; Zhang, Li Chun 1 ; Liu, Jian Qiang 1 ; He, Ming 1 ; Ma, Hui Hai 2 ; Yu, Yong Sheng 1 ; Cao, Yang 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Anim Biotechnol, 186 Dongxinghua St, Jilin 136100, Gongzhuling, Peoples R China
2.Jilin Acad Agr Sci, Inst Anim Husb & Vet Med, 186 Dongxinghua St, Jilin 136100, Gongzhuling, Peoples R China
关键词： SPARCL1; preadipocyte; proliferation; differentiation; apoptosis; sheep
期刊名称：ADIPOCYTE （ 影响因子：3.553； 五年影响因子：4.657 ）
ISSN： 2162-3945
年卷期： 2021 年 10 卷 1 期
页码：
收录情况： SCI
摘要： Important candidate genes that regulate lipid metabolism have the potential to increase the content of intramuscular fat (IMF) and improve meat quality. Secreted protein acidic and rich in cysteine like 1(SPARCL1) is a secreted glycoprotein with important physiological functions and is involved in the proliferation and differentiation of various cells. However, the role of the SPARCLI gene in sheep preadipocytes and its regulatory mechanism is still unclear. In this study, we explored the effect of SPARCL1 on the proliferation and differentiation of sheep preadipocytes. The results showed that the expression level of the SPARCL1 gene is higher in fat tissue than in other tissues, and the gene was significantly increased on the 6 th day of preadipocyte differentiation. In the preadipocyte proliferation stage, interference of SPARCL1 gene reduced cell viability and increased cell apoptosis. In preadipocyte differentiation stage, SPARCL1 overexpression significantly inhibited lipid droplets accumulation and triglyceride content by increasing Wnt10b, Fzd8, IL6, and beta-catenin and inhibiting PPAR gamma, C/E8P alpha, LPL, and IGF1 genes expression, whereas SPARCL1 deficiency significantly promoted cell differentiation by inhibiting beta-catenin and increasing GSK3P, PPARy, C/EBP alpha, and LPL. The results of this study suggest that SPARCL1 plays a negative role during preadipocyte differentiation and may become a novel target for regulating preadipocyte differentiation and improving IMF.
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