Quantitative H-1 NMR for the Direct Quantification of Saikosaponins in Bupleurum chinense DC.
文献类型： 外文期刊
作者： Zhao, Hua 1 ; Wang, Xiaohui 1 ; Zhang, Yuli 2 ; Huang, Xiaowan 3 ; Jiang, Yaling 1 ; Ma, Huan 4 ; An, Li 4 ; Wu, Xujin 4 ; W 1 ;
作者机构： 1.Zhengzhou Univ Ind Technol, Sch Pharm & Chem Engn, Zhengzhou 451100, Peoples R China
2.Henan Acad Sci, High & New Technol Res Ctr, Zhengzhou 450002, Peoples R China
3.Zhengzhou Univ, Inst Med & Pharmaceut Sci, Zhengzhou 450052, Peoples R China
4.Henan Acad Agr Sci, Inst Qual Standard & Testing Technol Agroprod, Key Lab Grain Qual & Safety & Testing Henan Prov, Zhengzhou 450002, Peoples R China
关键词： Bupleurum chinense DC; quantitative H-1 NMR; molality; saikosaponin; quality evaluation
期刊名称：ANALYTICAL SCIENCES （ 影响因子：2.081； 五年影响因子：1.676 ）
ISSN： 0910-6340
年卷期： 2021 年 37 卷 10 期
页码：
收录情况： SCI
摘要： Saikosaponin a and saikosaponin d are used as chemical standards for the quality evaluation of Bupleurum chinense DC. by the high-performance liquid chromatography method in current Chinese Pharmacopoeia. However, other saikosaponins, such as saikosaponin c and saikosaponin b(2), also possess pharmaceutical activity, but are not used as chemical standards. In this study, a quantitative proton nuclear magnetic resonance (H-1 NMR) method was developed to determine the total mass percentage (mg/g) of SSa, SSb1, SSb2 and SSd in B. chinense DC., using the H-24 (delta(H) 0.71) signal. Furthermore, the molality (mol/kg) of type I saikosaponins (epoxy-ether structure) was also determined by quantitative H-1 NMR in the area of H-11 (delta(H) 5.95) for a more accurate quality evaluation. Validation of the method confirmed that it has acceptable selectivity, precision, stability, and repeatability. The results indicated that this method has the potential to be a reliable method for the quantification of saikosaponins in Bupleurum scorzonerifolium Willd., vinegar baked B. chinense DC. and B. scorzonerifolium Willd., Chaihu Koufuye (oral liquid of Chaihu).
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