Hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract against carbon tetrachloride-induced hepatocyte damage in Cyprinus carpio
文献类型: 外文期刊
作者: Yin, Guojun 1 ; Cao, Liping 1 ; Xu, Pao 1 ; Jeney, Galina 2 ; Nakao, Miki 3 ;
作者机构: 1.Chinese Acad Fishery Sci, Key Open Lab Genet Breeding Aquat Animals & Aquac, Minist Agr, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
2.Res Inst Fisheries Aquaculture & Irrigat, H-4440 Szarvas, Hungary
3.Kyushu Univ, Marine Biochem Lab, Grad Sch Bioresource & Bioenvironm Sci, Fukuoka 8128581, Japan
关键词: Hibiscus sabdariffa extract;Carbon tetrachloride;Hepatoprotection;Antioxidant;Cyprinus carpio
期刊名称:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL ( 影响因子:2.416; 五年影响因子:2.117 )
ISSN: 1071-2690
年卷期: 2011 年 47 卷 1 期
页码:
收录情况: SCI
摘要: The present study aims to evaluate the hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract on the carbon tetrachloride (CCl(4))-induced hepatocyte damage in fish and provide evidence as to whether it can be potentially used as a medicine for liver diseases in aquaculture. H. sabdariffa extract (100, 200, and 400 mu g/mL) was added to the carp primary hepatocyte culture before (pre-treatment), after (post-treatment), and both before and after (pre- and post-treatment) the incubation of the hepatocytes with CCl(4). CCl(4) at 8 mM in the culture medium produced significantly elevated levels of lactate dehydrogenase (LDH), glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), and malondialdehyde (MDA) and significantly reduced levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Pre-treatment and pre- and post-treatment of the hepatocytes with H. sabdariffa extract significantly reduced the elevated levels of LDH, GOT, GPT, and MDA and increased the reduced activities of SOD and GSH-Px in a dose-dependent manner; post-treatment did not show any protective effect. The results suggest that H. sabdariffa extract can be potentially used for preventing rather than curing liver diseases in fish.
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