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Exogenous MgH2-derived hydrogen alleviates cadmium toxicity through m6A RNA methylation in rice

文献类型： 外文期刊

作者： Wang, Peiran 1 ; Cao, Junfeng 2 ; Lu, Jiayu 1 ; Xu, Xue 1 ; Wu, Shuang 1 ; Liu, Hongru 3 ; Wang, Xiufeng 1 ;

作者机构： 1.Anhui Acad Agr Sci, Rice Res Inst, Anhui Prov Key Lab Rice Germplasm Innovat & Mol Im, Hefei 230031, Peoples R China

2.Chinese Univ Hong Kong, Ctr Cell & Dev Biol, Sch Life Sci, State Key Lab Agrobiotechnol,Shatin, Hong Kong, Peoples R China

3.Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, 1000 Jingqi Rd, Shanghai 201403, Peoples R China

关键词： MgH2; Hydrogen; Cadmium stress; mRNA stability

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：11.3； 五年影响因子：12.4 ）

ISSN： 0304-3894

年卷期： 2024 年 480 卷

页码：

收录情况： SCI

摘要： Cadmium (Cd) contamination poses a substantial threat to crop yields and human health. While magnesium hydride (MgH2) has been reported as a hydrogen (H2) donor that promotes plant growth under heavy metal contamination, its role in rice remains elusive. Herein, seedlings of Oryza sativa L. Japonica variety Zhonghua 11 (ZH11) were selected and exposed to 20 mu L of 1-mol/L cadmium chloride (CdCl2) solution via hydroponics to simulate Cd stress. Meanwhile, 0.1 mg of MgH2 was used to slow-release H2 to the experimental group to explore its potential effects on rice over a 2-week period. The results indicated that Cd exposure severely inhibited the growth and development of ZH11 rice seedlings. However, the exogenous slow-release of H2 from MgH2 effectively mitigated this inhibitory effect by restoring the balance of reactive oxygen species (ROS), maintaining endogenous H2 homeostasis, and supporting the photosynthetic system. High-performance liquid chromatography analysis revealed that exogenous H2 reduces m6A RNA methylation levels in mRNA under Cd stress. Consequently, MeRIP-seq was conducted to investigate the effect of Cd exposure in rice in the presence and absence of H2. The m6A modifications were enriched at the start codon, stop codon, and 3 ' UTR. By integrating RNA-seq data, 118 transcripts were identified as differentially methylated and expressed genes under Cd stress. These gene annotations were associated with ROS, biological stress, and hormonal responses. Notably, 297 differentially methylated and expressed genes were identified under Cd stress in the presence of H2, linked to heavy metals, protein kinases, and calcium signaling regulation. Cd strongly activates the MAPK pathway in response to stress. Exogenous H2 reduces Cd accumulation as well as enhances plant tolerance and homeostasis by lowering m6A levels, thereby decreasing the mRNA stability of these genes. Our findings indicate that MgH2, by supplying H2, regulates gene expression through m6A RNA methylation and confers Cd tolerance in rice. This study provides potential candidate genes for studying the remediation of heavy metal pollution in plants.

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