文献类型： 外文期刊
作者： Lu, Xiaoguang 1 ; Zhang, Fuzhi 2 ; Zhang, Chenglong 1 ; Li, Guorui 1 ; Du, Yuchen 1 ; Zhao, Cicong 1 ; Zhao, Wei 1 ; Gao, Fengmei 3 ; Fu, Lianshuang 1 ; Liu, Xin 1 ; Liu, Jun 4 ; Wang, Xiaonan 1 ;
作者机构： 1.Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China
2.Harbin Inst Informat Engn, Harbin 150431, Peoples R China
3.Heilongjiang Acad Agr Sci, Crop Resources Inst, Harbin 150086, Peoples R China
4.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Resources & Genet Improvement, Beijing 100081, Peoples R China
关键词： Wheat; Tolerance cold; TaTPS11; Sucrose; Trehalose; CRISPR/Cas9
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）
ISSN： 0981-9428
年卷期： 2024 年 211 卷
页码：
收录情况： SCI
摘要： The presence of sugar in plant tissue can lead to an increase in the osmotic pressure within cells, a decrease in the freezing point of plants, and protection against ice crystal damage to the tissue. Trehalose is closely related to sucrose, which comprises the largest proportion of sugar and has become a hot topic of research in recent years. Our previous studies have confirmed that a key trehalose synthesis gene, TaTPS11, from the cold-resistant winter wheat DM1, could enhance the cold resistance of plants by increasing sugar content. However, the underlying mechanism behind this phenomenon remains unclear. In this study, we cloned TaTPS11-6D, edited TaTPS11-6D using CRISPR/Cas9 technology and transformed 'Fielder' to obtain T2 generation plants. We screened out OE3-3 and OE8-7 lines with significantly higher cold resistance than that of 'Fielder' and Cri 4-3 edited lines with significantly lower cold resistance than that of 'Fielder'. Low temperature storage limiting factors were measured for OE3-3, OE8-7 and Cri 4-3 treated at different temperatures.The results showed that TaTPS11-6D significantly increased the content of sugar in plants and the transfer of sugar from source to storage organs under cold conditions. The TaTPS11-6D significantly increased the levels of salicylic, jasmonic, and abscisic acids while also significantly decreasing the level of gibberellic acid. Our research improves the model of low temperature storage capacity limiting factor.
- 相关文献
作者其他论文 更多>>
-
Two telomere-to-telomere pig genome assemblies and pan-genome analyses provide insights into genomic structural landscape and genetic adaptations
作者：Zong, Wencheng;Chen, Li;Wang, Jinbu;Hou, Xinhua;Liu, Xin;Wang, Ligang;Wang, Lixian;Zhang, Longchao;Chen, Li;Chai, Jie;Wang, Jinyong;Zhang, Dongjie;Tian, Ming;He, Xinmiao;Liu, Di;Zhang, Yuebo;He, Jun;Yin, Yulong;Zhang, Yuebo;He, Jun;Yin, Yulong;An, Yalong;Song, Chengyi;D'Alessandro, Enrico;Li, Mingzhou
关键词：
-
Determination of future gully erosion risk and its spatially quantitative interpretability of driving factors in regional scale using machine learning algorithms
作者：Liu, Xin;Guo, Mingming;Zhang, Xingyi;Chen, Zhuoxin;Liu, Xin;Wang, Dichen;Wan, Zhaokai;Liu, Jielin
关键词：Gully erosion; Machine learning; GESM; Gully erosion risk; Northeast China
-
Shoot Apex Differentiation on Freezing Resistance and its Regulatory Genes in the Wheat Seedling Stage
作者：Zhang, Chenglong;Zhao, Wei;Li, Guorui;Zhao, Cicong;Yuan, Shuo;Fu, Lianshuang;Wang, Xiaonan;Zhang, Fuzhi;Peng, Kankan;Gao, Fengmei;Sun, Dan
关键词：freezing resistance; re-greening rate; shoot apex differentiation; wheat
-
The Impacts of Farming Activities on the Coevolutionary Structure of Plant Rhizosphere Soil Microbial Communities
作者：Wang, Qiuju;Liu, Xin;Zou, Jiahe;Li, Jingyang;Jiang, Yu;Mi, Gang;Guo, Zhenhua
关键词：Cyanobacteriales;
Fusarium ; plants;Rhizobia ; soil peroxidase -
Metabolomic Analysis of Maize Response to Northern Corn Leaf Blight
作者：Gu, Yingnan;Yang, Ye;Huang, Ying;Liu, Shubin;Yan, Bowei;Liu, Xin
关键词：
Zea mays L.; northern corn leaf blight; metabolomic; metabolic pathways -
Phosphorylation of Wild Soybean (Glycine soja) Splicing Factor GsSCL30a by GsSnRK1 Regulates Soybean Tolerance to Alkali Stress
作者：Li, Minglong;Liu, Xin;Zhao, Xin;Liu, Fujing;Xue, Man;Liu, Haishan;Ding, Xiaodong;Li, Qiang;Xiao, Jialei;Li, Minglong;Liu, Xin;Ding, Xiaodong;Li, Qiang;Xiao, Jialei;Sun, Xiaohuan;Liu, Changyuan;Liu, Zhangxiong
关键词：alkaline stress; cultivated soybean; GsSCL30a; splicing factor; wild soybean
-
Insights into the transcriptomic mechanism and characterization of endoglucanases from Aspergillus terreus in cellulose degradation
作者：Liu, Xin;Wang, Yanbo;Zhang, Rui;Hu, Xiaomei;Gao, Yunfei;Chen, Heshu;Dong, Shijia
关键词：Lignocellulose; Transcriptome; Endoglucanases; Molecular docking
