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Fine mapping of QPm.caas-3BS, a stable QTL for adult-plant resistance to powdery mildew in wheat (Triticum aestivum L.)

文献类型： 外文期刊

作者： Dong, Yan 1 ; Xu, Dengan 2 ; Xu, Xiaowan 3 ; Ren, Yan 4 ; Gao, Fengmei 5 ; Song, Jie 1 ; Jia, Aolin 1 ; Hao, Yuanfeng 1 ; He, Zhonghu 1 ; Xia, Xianchun 1 ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Natl Wheat Improvement Ctr, Inst Crop Sci, 12 Zhongguancun South St, Beijing 100081, Peoples R China

2.Qingdao Agr Univ, Coll Agron, Shandong Prov Key Lab Dryland Farming Technol, Qingdao 266109, Shandong, Peoples R China

3.Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China

4.Henan Agr Univ, Coll Agron, 63 Agr Rd, Zhengzhou 450002, Henan, Peoples R China

5.Heilongjiang Acad Agr Sci, Inst Crop Germplasm Resources, Harbin 150086, Heilongjiang, Peoples R China

6.CAAS, Int Maize & Wheat Improvement Ctr CIMMYT China Of, 12 Zhongguancun South St, Beijing 100081, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.574； 五年影响因子：5.662 ）

ISSN： 0040-5752

年卷期： 2022 年 135 卷 3 期

页码：

收录情况： SCI

摘要： Key message A stable QTL QPm.caas-3BS for adult-plant resistance to powdery mildew was mapped in an interval of 431 kb, and candidate genes were predicted based on gene sequences and expression profiles. Powdery mildew is a devastating foliar disease occurring in most wheat-growing areas. Characterization and fine mapping of genes for powdery mildew resistance can benefit marker-assisted breeding. We previously identified a stable quantitative trait locus (QTL) QPm.caas-3BS for adult-plant resistance to powdery mildew in a recombinant inbred line population of Zhou8425B/Chinese Spring by phenotyping across four environments. Using 11 heterozygous recombinants and high-density molecular markers, QPm.caas-3BS was delimited in a physical interval of approximately 3.91 Mb. Based on re-sequenced data and expression profiles, three genes TraesCS3B02G014800, TraesCS3B02G016800 and TraesCS3B02G019900 were associated with the powdery mildew resistance locus. Three gene-specific kompetitive allele-specific PCR (KASP) markers were developed from these genes and validated in the Zhou8425B derivatives and Zhou8425B/Chinese Spring population in which the resistance gene was mapped to a 0.3 cM interval flanked by KASP14800 and snp_50465, corresponding to a 431 kb region at the distal end of chromosome 3BS. Within the interval, TraesCS3B02G014800 was the most likely candidate gene for QPm.caas-3BS, but TraesCS3B02G016300 and TraesCS3B02G016400 were less likely candidates based on gene annotations and sequence variation between the parents. These results not only offer high-throughput KASP markers for improvement of powdery mildew resistance but also pave the way to map-based cloning of the resistance gene.

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