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Development and validation of high-throughput and low-cost STARP assays for genes underpinning economically important traits in wheat

文献类型： 外文期刊

作者： Wu, Yuying 1 ; Li, Ming 1 ; He, Zhonghu 1 ; Dreisigacker, Susanne 3 ; Wen, Weie 4 ; Jin, Hui 5 ; Zhai, Shengnan 7 ; Li, F 1 ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Natl Wheat Improvement Ctr, Inst Crop Sci, 12 Zhongguancun South St, Beijing 100081, Peoples R China

2.CAAS, Int Maize & Wheat Improvement Ctr CIMMYT China Of, 12 Zhongguancun South St, Beijing 100081, Peoples R China

3.Int Maize & Wheat Improvement Ctr CIMMYT, Global Wheat Program, Apdo Postal 6-641, Mexico City 06600, DF, Mexico

4.Zunyi Med Univ, Dept Cell Biol, 201 Dalian Rd, Zunyi 563099, Guizhou, Peoples R China

5.Heilongjiang Acad Agr Sci, Inst Forage & Grassland Sci, 368 Xuefu St, Harbin 150086, Heilongjiang, Peoples R China

6.Heilongjiang Acad Agr Sci, Crop Res Inst, 368 Xuefu St, Harbin 150086, Heilongjiang, Peoples R China

7.Shandong Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Wheat Biol & Genet Improvement Northern Y, Crop Res Inst,Natl Engn Lab Wheat & Maize, 202 Gongye North Rd, Jinan 250100, Shandong, Peoples R China

8.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, 7 Pengfei Rd, Shenzhen 518120, Guangdong, Peoples R China

9.Farm Seed Prod Gaoyi Cty, Gaoyi 051330, Hebei, Peoples R China

10.Anhui Acad Agr Sci, Crop Res Inst, 40 Nongke South St, Hefei 230001, Anhui, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2020 年 133 卷 8 期

页码：

收录情况： SCI

摘要： Key message We developed and validated 56 gene-specific semi-thermal asymmetric reverse PCR (STARP) markers for 46 genes of important wheat quality, biotic and abiotic stress resistance, grain yield, and adaptation-related traits for marker-assisted selection in wheat breeding. Development of high-throughput, low-cost, gene-specific molecular markers is important for marker-assisted selection in wheat breeding. In this study, we developed 56 gene-specific semi-thermal asymmetric reverse PCR (STARP) markers for wheat quality, tolerance to biotic and abiotic stresses, grain yield, and adaptation-related traits. The STARP assays were validated by (1) comparison of the assays with corresponding diagnostic STS/CAPS markers on 40 diverse wheat cultivars and (2) characterization of allelic effects based on the phenotypic and genotypic data of three segregating populations and 305 diverse wheat accessions from China and 13 other countries. The STARP assays showed the advantages of high-throughput, accuracy, flexibility, simple assay design, low operational costs, and platform compatibility. The state-of-the-art assays of this study provide a robust and reliable molecular marker toolkit for wheat breeding programs.

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