TaNAC100 acts as an integrator of seed protein and starch synthesis exerting pleiotropic effects on agronomic traits in wheat
文献类型： 外文期刊
作者： Li, Jihu 1 ; Xie, Lina 1 ; Tian, Xiuling 1 ; Liu, Siyang 1 ; Xu, Dengan 1 ; Jin, Hui 3 ; Song, Jie 1 ; Dong, Yan 1 ; Zhao, D 1 ;
作者机构： 1.Chinese Acad Agr Sci CAAS, Natl Wheat Improvement Ctr, Inst Crop Sci, 12 Zhongguancun South St, Beijing 100081, Peoples R China
2.Shandong Acad Agr Sci, Crop Res Inst, 202 Gongysse North St, Jinan 250100, Shandong, Peoples R China
3.Heilongjiang Acad Agr Sci, Inst Forage & Grassland Sci, 368 Xuefu St, Harbin 150086, Heilongjiang, Peoples R China
4.Int Maize & Wheat Improvement Ctr CIMMYT, China Off, 12 Zhongguancun South St, Beijing 100081, Peoples R China
关键词： GLU-1; HMW-GS; seed storage protein; starch; TaNAC100; transcription factor; wheat
期刊名称：PLANT JOURNAL （ 影响因子：6.486； 五年影响因子：7.666 ）
ISSN： 0960-7412
年卷期： 2021 年 108 卷 3 期
页码：
收录情况： SCI
摘要： High-molecular-weight glutenin subunits (HMW-GS) are major components of seed storage proteins (SSPs) and largely determine the processing properties of wheat (Triticum aestivum) flour. HMW-GS are encoded by the GLU-1 loci and regulated at the transcriptional level by interaction between cis-elements and transcription factors (TFs). We recently validated the function of conserved cis-regulatory modules (CCRMs) in GLU-1 promoters, but their interacting TFs remained uncharacterized. Here we identified a CCRM-binding NAM-ATAF-CUC (NAC) protein, TaNAC100, through yeast one-hybrid (Y1H) library screening. Transactivation assays demonstrated that TaNAC100 could bind to the GLU-1 promoters and repress their transcription activity in tobacco (Nicotiana benthamiana). Overexpression of TaNAC100 in wheat significantly reduced the contents of HMW-GS and other SSPs as well as total seed protein. This was confirmed by transcriptome analyses. Conversely, enhanced expression of TaNAC100 increased seed starch contents and expression of key starch synthesis-related genes, such as TaGBSS1 and TaSUS2. Y1H assays also indicated TaNAC100 binding with the promoters of TaGBSS1 and TaSUS2. These results suggest that TaNAC100 functions as a hub controlling seed protein and starch synthesis. Phenotypic analyses showed that TaNAC100 overexpression repressed plant height, increased heading date, and promoted seed size and thousand kernel weight. We also investigated sequence variations in a panel of cultivars, but did not identify significant association of TaNAC100 haplotypes with agronomic traits. The findings not only uncover a useful gene for wheat breeding but also provide an entry point to reveal the mechanism underlying metabolic balance of seed storage products.
- 相关文献
作者其他论文 更多>>
-
A comparison of Chinese soybean collection with European soybean collections on genetic diversity by genome-wide scan
作者：Xu, Jiangyuan;Lu, Yuqing;Gu, Yongzhe;Gao, Huawei;Liu, Zhangxiong;Qiu, Lijuan;Yao, Xindong;Yao, Xindong;Leopold, Rittler;Yuan, Ming;Zhang, Yong;Sun, Rujian;Xue, Yongguo;Liu, Yeli;Han, Dezhi;Wang, Jinxing;Qiu, Lijuan
关键词：Chinese soybean; European soybean; Genetic diversity; Selective sweep analysis; Selected region
-
Soybean yield estimation and lodging classification based on UAV multi-source data and self-supervised contrastive learning
作者：Zhou, Longyu;Chen, Haochong;Feng, Puyu;Ma, Yuntao;Zhang, Yong;Wang, Lei;Li, Mingxue;Sun, Xuhong;Sun, Guangyao;Yan, Long;Qiu, Lijuan;Li, Yinghui
关键词：Remote sensing; 3D reconstruction; Point cloud; Multispectral; Structure from motion
-
Bridging the gap between hyperspectral imaging and crop breeding: soybean yield prediction and lodging classification with prototype contrastive learning
作者：Sun, Guangyao;Han, Shiteng;Xu, Yun;Ma, Yuntao;Zhang, Yong;Wang, Lei;Zhou, Longyu;Fei, Shuaipeng;Xiao, Shunfu;Ma, Yuntao;Yan, Long;Che, Yingpu;Li, Yinghui;Qiu, Lijuan
关键词：Unmanned Aerial Vehicle; Deep Learning; Resnet; Soybean Yield and Lodging Prediction
-
Volatilomic profiling of controlled atmosphere stored blue honeysuckle fruit using GC-MS, E-nose, and GC-IMS
作者：Huo, Junwei;Sui, Xiaonan;Zhang, Yan;Huo, Junwei;Zhang, Yan;Xiao, Zhen;Sun, Jinhui;Huo, Junwei;Zhang, Yan;Sui, Xiaonan;Lan, Yaqi;Jing, Qiuju;Xie, Shuangyu;Che, Jianing
关键词：Haskap fruits; Postharvest; Volatiles; GC-MS; GC-IMS
-
Characterization of a Natural Accession of Elymus sibiricus with In Situ Hybridization and Agronomic Evaluation
作者：Liu, Yizhuo;Ding, Jiarui;Wu, Chunfei;Song, Weiwei;Zhao, Xinyu;Li, Mingyao;Yan, Xinyu;Zhu, Liangyu;Bao, Yaqi;Liu, Dianhao;Li, Xinling;Zhang, Yanming;Zhao, Haibin;Jin, Hui;Zhang, Rui;Qu, Yunfeng;Li, Hongjie;Cui, Lei
关键词：
Elymus ; smGISH; karyotype; chromosome; cold-hardiness -
Soybean Lodging Classification and Yield Prediction Using Multimodal UAV Data Fusion and Deep Learning
作者：Xu, Xingmei;Fang, Yushi;Yu, Helong;Ma, Yuntao;Sun, Guangyao;Guo, Yan;Ma, Yuntao;Zhang, Yong;Wang, Lei;Chen, Chen;Ren, Lisuo;Meng, Lei;Li, Yinghui;Qiu, Lijuan
关键词：UAV; deep learning; lodging classification; yield; data fusion
-
Loss of phytochromobilin synthase activity leads to larger seeds with higher protein content in soybean
作者：Su, Xin;Han, Ying-Peng;Su, Xin;Hong, Hui-Long;Qiu, Li-juan;Wang, Hao-Rang;Zhang, Yong;Sun, Xu-hong;Wang, Lei;Song, Ji-Ling;Yang, Meng-Ping;Yang, Xing-Yong
关键词：GmYGL2; QTL; Seed size; Seed weight; Soybean
