文献类型： 外文期刊

作者： Liu, Xing ¹ ; Lu, Xiang ¹ ; Yang, Sheng ¹ ; Liu, Yu ¹ ; Wang, Wenqing ² ; Wei, Xin ¹ ; Ji, Hongjiu ³ ; Zhang, Bo ⁴ ; Xin, Wenzhen ¹ ; Wen, Junxiu ¹ ; Wang, Jinwang ¹ ; Chen, Qiuxia ¹ ;

作者机构： 1.Zhejiang Inst Subtrop Crops, Zhejiang Acad Agr Sci, Wenzhou Key Lab Resource Plant Innovat & Utilizat, Wenzhou 325005, Zhejiang, Peoples R China

2.Xiamen Univ, Coll Environm & Ecol, Key Lab Minist Educ Coastal & Wetland Ecosyst, Xiamen 361102, Fujian, Peoples R China

3.Marine Fisheries Res Inst Jiangsu Prov, Nantong 226007, Jiangsu, Peoples R China

4.Wenzhou Kean Univ, Wenzhou Municipal Key Lab Appl Biomed & Biopharmac, Wenzhou 325060, Zhejiang, Peoples R China

5.Wenzhou Univ, Coll Life & Environm Sci, Wenzhou 325000, Zhejiang, Peoples R China

关键词： Kandelia obovata; Cold stress; Abscisic acid; Physiological responses; Transcriptomic analysis; Phenylpropanoid metabolism

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.26； 五年影响因子：5.761 ）

ISSN： 1471-2229

年卷期： 2022 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background: Mangroves possess substantial ecological, social, and economic functions in tropical and subtropical coastal wetlands. Kandelia obovata is the most cold-resistance species among mangrove plants, with a widespread distribution in China that ranges from Sanya (18 degrees 12' N) to Wenzhou (28 degrees 20' N). Here, we explored the temporal variations in physiological status and transcriptome profiling of K. obovata under natural frost conditions at similar to 32 degrees N, as well as the positive role of exogenous abscisic acid (ABA) in cold resistance. Results: The soluble sugar (SS) and proline (Pro) functioned under freezing stress, of which SS was more important for K. obovata. Consistently, up-regulated DEGs responding to low temperature were significantly annotated to glycometabolism, such as starch and sucrose metabolism and amino sugar and nucleotide sugar metabolism. Notably, the top 2 pathways of KEGG enrichment were phenylpropanoid biosynthesis and flavonoid biosynthesis. For the antioxidant system, POD in conjunction with CAT removed hydrogen peroxide, and CAT appeared to be more important. The up-regulated DEGs responding to low temperature and ABA were also found to be enriched in arginine and proline metabolism, starch and sucrose metabolism, and peroxisome. Moreover, ABA triggered the expression of P5CS and P5CR, but inhibited the ProDH expression, which might contribute to Pro accumulation. Interestingly, there was no significant change in malondialdehyde (MDA) content during the cold event (P> 0.05), suggesting foliar application of ABA effectively alleviated the adverse effects of freezing stress on K. obovata by activating the antioxidant enzyme activity and increasing osmolytes accumulation, such as Pro, and the outcome was proportional to ABA concentration. Conclusions: This study deepened our understanding of the physiological characters and molecular mechanisms underlying the response of K. obovata to natural frost conditions and exogenous ABA at the field level, which could provide a sound theoretical foundation for expanding mangroves plantations in higher latitudes, as well as the development coastal landscape.