您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Isolation, characterization, and genome analysis of bacteriophage P929 that could specifically lyase the KL19 capsular type of Klebsiella pneumoniae

文献类型： 外文期刊

作者： Chen, Xinyi 1 ; Tang, Qi 1 ; Li, Xue 5 ; Zheng, Xiangkuan 1 ; Li, Pei 1 ; Li, Min 1 ; Wu, Fei 5 ; Xu, Zhengjun 6 ; Lu, Renfei 5 ; Zhang, Wei 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, 1 Weigang, Nanjing 210095, Peoples R China

2.Minist Agr, Key Lab Anim Bacteriol, 1 Weigang, Nanjing 210095, Peoples R China

3.OIE Reference Lab Swine Streptococcosis, 1 Weigang, Nanjing 210095, Peoples R China

4.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, 1 Weigang, Nanjing 210095, Peoples R China

5.Nantong Univ, Dept Clin Lab, Nantong Third Hosp, Nantong 212006, Jiangsu, Peoples R China

6.Jiangsu Anim Dis Control Ctr, 124 Caochangmen St, Nanjing 210036, Peoples R China

关键词： Phage; Phage therapy; Klebsiella pneumoniae; Carbapenem-resistant; Genome analysis

期刊名称：VIRUS RESEARCH （ 影响因子：6.286； 五年影响因子：4.486 ）

ISSN： 0168-1702

年卷期： 2022 年 314 卷

页码：

收录情况： SCI

摘要： In recent years, Klebsiella pneumoniae has caused an increase in the number of serious infections associated with pneumonia, septicemia, urinary tract infections, and pyogenic liver abscess. In this study, a phage P929, isolated from hospital sewage in Jiangsu, could specifically infect K. pneumoniae KL19 capsular type by forming plaques with a translucent halo that expanded over time. Phage P929 with a multiplicity of infection (MOI) of 0.1 produced the highest phage titer. According to a one-step growth curve experiment, the latent time period of phage P929 was 25 min, and the burst size was about 156 phage particles/cell. The sensitivity tests confirmed that P929 was stable at temperatures ranging from 4 to 50 ? and pH 3 to 11. Based on morphological obser-vation and phylogenetic analysis, phage P929 could be assigned to a new species in the genus Drulisvirus of the subfamily Slopekvirinae in the family Autographiviridae. According to genome analysis, phage P929 was 44,764 bp in size with 53.66% G + C content, encoding 57 proteins or coding sequences (117-3699 bp in length). Phage P929 showed potential antibacterial activity on planktonic cells and biofilm. After 120 min, the OD600 values of five phage-treated groups were basically reached zero compared to the untreated group, and the antibacterial activity of P929 was still detectable within 390 min. In anti-biofilm tests, phage P929 at an MOI of 1 significantly reduced the biofilm formation of K. pneumoniae in 48 h. These results suggest that phage P929 may be used to treat carbapenem-resistant and biofilm-forming K. pneumonia in clinical settings.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台