The glycosylation sites in RBD of spike protein attenuate the immunogenicity of PEDV AH2012/12
文献类型： 外文期刊
作者： Zhang, Gege 1 ; Peng, Qi 2 ; Liu, Shiyu 2 ; Fan, Baochao 2 ; Wang, Chuanhong 2 ; Song, Xu 2 ; Cao, Qiuxia 2 ; Li, Chengcheng 2 ; Xu, Hong 2 ; Lu, Hongting 2 ; Bao, Meiying 2 ; Yang, Shanshan 2 ; Li, Yunchuan 2 ; Wang, Jiaxiang 1 ; Li, Bin 2 ;
作者机构： 1.Yangtze Univ, Coll Anim Sci, Jingzhou 434025, Peoples R China
2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol,Minist Agr,Inst Vet Med,Key L, Nanjing 210014, Jiangsu, Peoples R China
3.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China
4.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Jiangsu Key Lab Zoonoses, Yangzhou 225009, Jiangsu, Peoples R China
5.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Jiangsu, Peoples R China
关键词： PEDV; RBD; Glycosylation; Reverse genetics; Pathogenicity; Immunogenicity
期刊名称：VIRUS RESEARCH （ 影响因子：2.5； 五年影响因子：3.2 ）
ISSN： 0168-1702
年卷期： 2024 年 345 卷
页码：
收录情况： SCI
摘要： Porcine epidemic diarrhea (PED) is a highly contagious swine intestinal disease caused by PED virus (PEDV). Vaccination is a promising strategy to prevent and control PED. Previous studies have confirmed that glycosylation could regulate the immunogenicity of viral antigens. In this study, we constructed three recombinant PEDVs which removed the glycosylation sites in RBD. Viral infection assays revealed that similar replication characteristics between the recombinant viruses and parental PEDV. Although animal challenging study demonstrated that the glycosylation sites in RBD do not affect the pathogenicity of PEDV, we found that removing the glycosylation sites on the RBD regions could promote the IgG and neutralization titer in vivo, suggesting deglycosylation in RBD could enhance the immunogenicity of PEDV. These findings demonstrated that removal of the glycosylation sites in RBD is a promising method to develop PEDV vaccines.
- 相关文献
作者其他论文 更多>>
-
Inhibition of Hyperglycemia and Hyperlipidemia by Blocking Toll-like Receptor 4: Comparison of Wild-Type and Toll-like Receptor 4 Gene Knockout Mice on Obesity and Diabetes Modeling
作者：Zhao, Xingyu;Huang, Wuyang;Zhao, Xingyu;Zheng, Jiawei;Huang, Wuyang;Wang, Jing;Huang, Wuyang;Li, Bin
关键词：TLR4; glycolipid metabolism; obesity and diabetes modeling; knockout
-
Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis
作者：Sun, Chengming;Zhou, Xiaoying;Fu, Sanxiong;Wang, Xiaodong;Peng, Qi;Gao, Jianqin;Chen, Feng;Zhang, Wei;Hu, Maolong;Zhang, Jiefu;Wu, Jian;Liu, Huimin;Wang, Youping;Xue, Zhifei;Fu, Tingdong;Yi, Bin
关键词：Rapeseed; Branch angle; Homoeologous exchange; WGCNA; QTL-seq; WRKY40
-
Lipidomics reveals the significance and mechanism of the cellular ceramide metabolism for rotavirus replication
作者：Tao, Ran;Cheng, Xi;Gu, Laqiang;Zhou, Jinzhu;Zhu, Xuejiao;Zhang, Xuehan;Guo, Rongli;Wang, Wei;Li, Bin;Tao, Ran;Cheng, Xi;Gu, Laqiang;Zhou, Jinzhu;Zhu, Xuejiao;Zhang, Xuehan;Guo, Rongli;Wang, Wei;Li, Bin;Tao, Ran;Cheng, Xi;Gu, Laqiang;Zhou, Jinzhu;Zhu, Xuejiao;Zhang, Xuehan;Guo, Rongli;Wang, Wei;Li, Bin;Cheng, Xi;Li, Bin;Gu, Laqiang;Li, Bin
关键词：rotavirus; lipidomics; ceramide; inhibition; viral replication; apoptosis
-
First Specific Detection of Mammalian Orthoreovirus from Goats Using TaqMan Real-Time RT-PCR Technology
作者：Mao, Li;Li, Xia;Cai, Xuhang;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Mao, Li;Li, Xia;Cai, Xuhang;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Mao, Li;Li, Xia;Cai, Xuhang;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Mao, Li;Li, Xia;Cai, Xuhang;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Mao, Li;Li, Xia;Li, Wenliang;Li, Jizong;Suolang, Sizhu;Li, Bin;Mao, Li;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Cai, Xuhang;Zhai, Junjun
关键词：mammalian orthoreovirus; TaqMan qRT-PCR; detection; goat; sheep
-
A Novel Polymer Nanoparticle Polydimethyl Diallyl Ammonium Chloride as An Adjuvant Enhances the Immune Response of SARS-CoV-2 Subunit Vaccine
作者：Ren, Lili;Ouyang, Chengcheng;Zhao, Shuqing;Zheng, Qiqi;Guo, Weilu;Zhang, Wei;Zhao, Shuqing;Zheng, Qiqi;Guo, Weilu;Fan, Baochao;Zhou, Jinzhu;Hu, Mi;Li, Jizong;Li, Bin;Fan, Baochao;Li, Jizong;Li, Bin;Fan, Baochao;Li, Jizong;Li, Bin;Fan, Baochao;Li, Jizong;Li, Bin
关键词：adjuvant; polydimethyl diallyl ammonium chloride; SARS-CoV-2; subunit vaccine
-
The synergy of recombinant NSP4 and VP4 from porcine rotavirus elicited a strong mucosal response
作者：Li, Sufen;Tang, Xuechao;Zhou, Jinzhu;Bian, Xianyu;Wang, Jianxin;Gu, Laqiang;Zhu, Xuejiao;Tao, Ran;Sun, Min;Zhang, Xuehan;Li, Bin;Li, Sufen;Zhou, Jinzhu;Zhu, Xuejiao;Sun, Min;Zhang, Xuehan;Li, Bin;Zhou, Jinzhu;Zhu, Xuejiao;Sun, Min;Zhang, Xuehan;Li, Bin;Li, Bin
关键词：Rotavirus; Porcine; Mucosal immunity; NSP4; VP4
-
Comparative transcriptome reveals EphA2 and c-Fos as key factors driving enhanced replication in high-passage porcine deltacoronavirus strain
作者：Liu, Shiyu;Fan, Baochao;Zhang, Gege;He, Wenlong;Wang, Chuanhong;Xie, Jingyuan;Song, Xu;Yuan, Boshui;Guo, Rongli;Li, Jizong;Li, Bin;Liu, Shiyu;Fan, Baochao;Li, Bin;Peng, Qi;Fan, Baochao;Li, Bin
关键词：PDCoV; LLC-PK1; RNA-Seq; MAPK pathway; EphA2; C-Fos
