The glycosylation sites in RBD of spike protein attenuate the immunogenicity of PEDV AH2012/12
文献类型： 外文期刊
作者： Zhang, Gege 1 ; Peng, Qi 2 ; Liu, Shiyu 2 ; Fan, Baochao 2 ; Wang, Chuanhong 2 ; Song, Xu 2 ; Cao, Qiuxia 2 ; Li, Chengcheng 2 ; Xu, Hong 2 ; Lu, Hongting 2 ; Bao, Meiying 2 ; Yang, Shanshan 2 ; Li, Yunchuan 2 ; Wang, Jiaxiang 1 ; Li, Bin 2 ;
作者机构： 1.Yangtze Univ, Coll Anim Sci, Jingzhou 434025, Peoples R China
2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol,Minist Agr,Inst Vet Med,Key L, Nanjing 210014, Jiangsu, Peoples R China
3.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China
4.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Jiangsu Key Lab Zoonoses, Yangzhou 225009, Jiangsu, Peoples R China
5.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Jiangsu, Peoples R China
关键词： PEDV; RBD; Glycosylation; Reverse genetics; Pathogenicity; Immunogenicity
期刊名称：VIRUS RESEARCH （ 影响因子：2.5； 五年影响因子：3.2 ）
ISSN： 0168-1702
年卷期： 2024 年 345 卷
页码：
收录情况： SCI
摘要： Porcine epidemic diarrhea (PED) is a highly contagious swine intestinal disease caused by PED virus (PEDV). Vaccination is a promising strategy to prevent and control PED. Previous studies have confirmed that glycosylation could regulate the immunogenicity of viral antigens. In this study, we constructed three recombinant PEDVs which removed the glycosylation sites in RBD. Viral infection assays revealed that similar replication characteristics between the recombinant viruses and parental PEDV. Although animal challenging study demonstrated that the glycosylation sites in RBD do not affect the pathogenicity of PEDV, we found that removing the glycosylation sites on the RBD regions could promote the IgG and neutralization titer in vivo, suggesting deglycosylation in RBD could enhance the immunogenicity of PEDV. These findings demonstrated that removal of the glycosylation sites in RBD is a promising method to develop PEDV vaccines.
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