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Scalable biomimetic sensing system with membrane receptor dual-monolayer probe and graphene transistor arrays

文献类型： 外文期刊

作者： Qing, Rui 1 ; Xue, Mantian 4 ; Zhao, Jiayuan 5 ; Wu, Lidong 6 ; Breitwieser, Andreas 7 ; Smorodina, Eva 8 ; Schubert, Thomas 10 ; Azzellino, Giovanni 5 ; Jin, David 11 ; Kong, Jing 5 ; Palacios, Tomas 4 ; Sleytr, Uwe B. 7 ; Zhang, Shuguang 2 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

2.MIT, MIT Media Lab, 77 Massachusetts Ave, Cambridge, MA 02139 USA

3.MIT, David H Koch Inst Integrat Canc Res, 77 Massachusetts Ave, Cambridge, MA 02139 USA

4.MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02142 USA

5.MIT, Res Lab Elect, Cambridge, MA 02139 USA

6.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China

7.Univ Nat Resources & Life Sci, Dept Bionanosciences DBNS, BOKU, Vienna, Austria

8.Univ Oslo, Dept Immunol, Oslo, Norway

9.Oslo Univ Hosp, Oslo, Norway

10.2bind GmbH, Biopk 11, D-93053 Regensburg, Germany

11.Avalon GloboCare Corp, Freehold, NJ 07728 USA

期刊名称：SCIENCE ADVANCES （ 2022影响因子：13.6； 五年影响因子：15.4 ）

ISSN： 2375-2548

年卷期： 2023 年 9 卷 29 期

页码：

收录情况： SCI

摘要： Affinity-based biosensing can enable point-of-care diagnostics and continuous health monitoring, which commonly follows bottom-up approaches and is inherently constrained by bioprobes' intrinsic properties, batch-to-batch consistency, and stability in biofluids. We present a biomimetic top-down platform to circumvent such difficulties by combining a "dual-monolayer" biorecognition construct with graphene-based field-effect-transistor arrays. The construct adopts redesigned water-soluble membrane receptors as specific sensing units, positioned by two-dimensional crystalline S-layer proteins as dense antifouling linkers guiding their orientations. Hundreds of transistors provide statistical significance from transduced signals. System feasibility was demonstrated with rSbpA-ZZ/CXCR4(QTY)-Fc combination. Nature-like specific interactions were achieved toward CXCL12 ligand and HIV coat glycoprotein in physiologically relevant concentrations, without notable sensitivity loss in 100% human serum. The construct is regeneratable by acidic buffer, allowing device reuse and functional tuning. The modular and generalizable architecture behaves similarly to natural systems but gives electrical outputs, which enables fabrication of multiplex sensors with tailored receptor panels for designated diagnostic purposes.

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