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Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain

文献类型： 外文期刊

作者： Sun, Yun 1 ; Liu, Chun-sheng 1 ; Sun, Li 1 ;

作者机构： 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： Edwardsiella tarda;Antigen;Subunit vaccine;Surface display

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2010 年 28 卷 40 期

页码：

收录情况： SCI

摘要： Edwardsiella tarda is the etiological agent of edwardsiellosis, a systematic disease that affects a wide range of marine and freshwater fish cultured worldwide. In order to identify E. tarda antigens with vaccine potential, we in this study conducted a systematic search for E. tarda proteins with secretion capacity. One of the proteins thus identified was Esa1, which contains 795 amino acid residues and shares extensive overall sequence identities with the D15-like surface antigens of several bacterial species. In silico analyses indicated that Esa1 localizes to outer membrane and possesses domain structures that are conserved among bacterial surface antigens. The vaccine potential of purified recombinant Esa1 was examined in a Japanese flounder (Paralichthys olivaceus) model, which showed that fish vaccinated with Esa1 exhibited a high level of survival and produced specific serum antibodies. Passive immunization of naive fish with antisera raised against Esa1 resulted in significant protection against E. tarda challenge. Taking advantage of the secretion capacity of Esa1 and the natural gut-colonization ability of a fish commensal strain, we constructed an Esa1-expressing recombinant strain, FP3/pJsa1. Western immunoblot and agglutination analyses showed that FP3/pJsa1 produces outer membrane-localized Esa1 and forms aggregates in the presence of anti-Esa1 antibodies. Vaccination analyses showed that FP3/pJsa1 as an intraperitoneal injection vaccine and an oral vaccine embedded in alginate microspheres produced relative percent survival rates of 79% and 52%, respectively, under severe challenging conditions that resulted in 92-96% mortality in control fish. Further analyses showed that following oral vaccination, FP3/pJsa1 was able to colonize in the gut but unable to disseminate into other tissues. Together these results indicate that Esa1 is a protective immunogen and an effective oral vaccine when delivered by FP3/pJsa1 as a surface-anchored antigen. (c) 2010 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Comparative study of the immune effect of an Edwardsiella tarda antigen in two forms: Subunit vaccine vs DNA vaccine. Sun, Yun,Sun, Li,Sun, Yun,Liu, Chun-Sheng.

[2]Proteome profiling reveals immune responses in Japanese flounder (Paralichthys olivaceus) infected with Edwardsiella tarda by iTRAQ analysis. Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin,Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin.

[3]Regulation of the Edwardsiella tarda Hemolysin Gene and luxS by EthR. Wang Fang,Zhang, Min,Hu, Yong-hua,Zhang, Wei-wei,Sun, Li,Wang Fang,Zhang, Min,Hu, Yong-hua,Zhang, Wei-wei,Wang Fang.

[4]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[5]A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Xie Guosi,Huang Jie. 2012

[6]Specific MHC class II B alleles associated with resistance to Edwardsiella tarda in turbot, Psetta maxima (L.). Xu, J-Y,Chen, S-L,Ding, H.,Xu, J-Y. 2009

[7]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[8]Isolation and analysis of the vaccine potential of an attenuated Edwardsiella tarda strain. Sun, Yun,Liu, Chun-sheng,Sun, Li,Sun, Yun,Liu, Chun-sheng. 2010

[9]A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection. Sun, Y.,Hu, Y-H.,Sun, L.,Sun, Y.,Liu, C-S.. 2012

[10]Optimization of protectant, salinity and freezing condition for freeze-drying preservation of Edwardsiella tarda. Yu, Yongxiang,Xue, Liangyi,Yu, Yongxiang,Zhang, Zheng,Wang, Yingeng,Liao, Meijie,Li, Bin. 2017

[11]MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families. Du Min,Chen SongLin,Yang JingFeng,Zhang Bo,Du Min,Liu YanHong,Niu BaoZhen,Du Min. 2012

[12]Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant. Mo, Zhao-Lan,Li, Jie,Li, Gui-Yang,Xiao, Peng. 2013

[13]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[14]Domain analysis of the Edwardsiella tarda ferric uptake regulator. Sun, Kun,Cheng, Shuang,Wang, Fang,Sun, Li,Sun, Kun,Cheng, Shuang,Wang, Fang,Wang, Fang.

[15]Generation and evaluation of virulence attenuated mutants of Edwardsiella tarda as vaccine candidates to combat edwardsiellosis in flounder (Paralichthys olivaceus). Li, Jie,Mo, Zhaolan,Li, Guiyang,Huang, Jie,Li, Jie,Mo, Zhaolan,Li, Guiyang,Huang, Jie,Xiao, Peng.

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