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Development of MHC Class I and II B Primers in Common Carp and its Molecular Characterization

文献类型: 外文期刊

作者: Jia, Zhiying 1 ; Chi, Xifeng 1 ; Li, Chitao 1 ; Shi, Lianyu 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词: MHC;Common carp;Polymorphic;Variable nucleotide site

期刊名称:BIOCHEMICAL GENETICS ( 影响因子:1.89; 五年影响因子:1.959 )

ISSN: 0006-2928

年卷期: 2010 年 48 卷 7-8 期

页码:

收录情况: SCI

摘要: The major histocompatibility complex (MHC) has an important role in immune response and is known as the most polymorphic locus in vertebrates. We developed three pairs of polymerase chain reaction primers of the alpha-2 domain (exon 3) of MHC class I and the beta-2 (exon 3) and beta-3 domains (exon 4) of MHC class II B gene in the German mirror common carp (Cyprinus carpio L.). We analyzed the three loci in a population of 65 individuals that had suffered the serious disease of gill rot. Five to six variable nucleotide sites and two to six variable amino acid sites (71.43%) were detected in the exon sequence of the sampled populations, indicating that many of them corresponded to amino acids involved in antigen recognition. Deviation from Hardy-Weinberg equilibrium and linkage disequilibrium were differentially found in some loci, which will be important for further study of disease resistance/susceptibility and population evolution.

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