文献类型： 外文期刊
作者： Hong, Yuhui 1 ; Zhang, Yuanyuan 1 ; Cui, Jun 1 ; Meng, Jun 4 ; Chen, Yinhua 5 ; Zhang, Chengwei 6 ; Yang, Jinxiao 6 ; Luan, Yushi 1 ;
作者机构： 1.Dalian Univ Technol, Sch Bioengn, Dalian 116024, Peoples R China
2.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 311400, Peoples R China
3.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Peoples R China
4.Dalian Univ Technol, Sch Comp Sci & Technol, Dalian 116024, Peoples R China
5.Hainan Univ, Coll Trop Crops, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou 570228, Hainan, Peoples R China
6.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Maize DNA Fingerprinting & Mol Br, Beijing 100000, Peoples R China
关键词： disease resistance; lncRNA; miRNA; Phytophthora infestans; target gene; tomato
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.106； 五年影响因子：8.241 ）
ISSN： 1672-9072
年卷期： 2022 年 64 卷 10 期
页码：
收录情况： SCI
摘要： The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans. The long non-coding RNA (lncRNA) Sl-lncRNA39896 is induced after P. infestans infection and was previously predicted to act as an endogenous target mimic (eTM) for the microRNA Sl-miR166b, which function in stress responses. Here, we further examined the role of Sl-lncRNA39896 and Sl-miR166b in tomato resistance to P. infestans. Sl-miR166b levels were higher in Sl-lncRNA39896-knockout mutants than in wild-type plants, and the mutants displayed enhanced resistance to P. infestans. A six-point mutation in the region of Sl-lncRNA39896 that binds to Sl-miR166b disabled the interaction, suggesting that Sl-lncRNA39896 acts as an eTM for Sl-miR166b. Overexpressing Sl-miR166b yielded a similar phenotype to that produced by Sl-lncRNA39896-knockout, whereas silencing of Sl-miR166b impaired resistance. We verified that Sl-miR166b cleaved transcripts of its target class III homeodomain-leucine zipper genes SlHDZ34 and SlHDZ45. Silencing of SlHDZ34/45 decreased pathogen accumulation in plants infected with P. infestans. Additionally, jasmonic acid and ethylene contents were elevated following infection in the plants with enhanced resistance. Sl-lncRNA39896 is the first known lncRNA to negatively regulate resistance to P. infestans in tomato. We propose a novel mechanism in which the lncRNA39896-miR166b-HDZ module modulates resistance to P. infestans.
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